Results of Chemical and Pharmacological Studies of the Mongolian Traditional Prescription "Arur-10"

Nyamdemberel Tsagaanbaatar^1*, Uranzaya Dashzeveg¹, Nomindari Nyamdemberel², Myadagbadam Urtnasan¹ and Chimedragchaa Chimedtseren¹

¹Institute of Traditional Medicine and Technology, Ulaanbaatar, Mongolia

 ²Mongolia National University of Medical Sciences, Ulaanbaatar, Mongolia.

Corresponding Author E-mail: nyamaa071427@gmail.com

Abstract: This study examines ARUR-10, the herbal prescription commonly utilized in Traditional Mongolian Medicine practices for the treatment of kidney disease and the improvement of physical strength. This investigation aims to establish a profile of the biologically active compounds, antioxidant activity, and the anti-inflammatory impact on the kidneys in the traditional ARUR-10 prescription. For this analysis, we used Thin-Layer Chromatography (TLC) to identify phenolic compounds in the traditional prescription. Quantification of these bioactive substances was determined by UV/Vis spectrophotometry. The traditional ARUR-10 prescription, or traditional drug, had a higher polyphenolic content of 12.9±0.361% (gallic acid equivalent) and a sum flavonoid content of 0.33±0.015% (Rutin equivalent). The antioxidant activity was measured using the inhibiting free radical’s DPPH method and evaluated using the IC50 value. The 100 μg/ml concentration inhibited free radicals by 68.47%, whereas 200 μg/ml inhibited them by 83.29%, with an IC50 of 92.4 μg/ml. This study concluded that the traditional ARUR-10 prescription contains many polyphenolic compounds and flavonoids and has antioxidant properties. ARUR-10 has a moderate level of toxicity and no signs of chronic toxicity have been detected. Urine excretion was 1.52 ± 1.23 times higher than in the distilled water group (0.97 ± 0.71). ARUR-10 at doses of 240 mg/kg (1.62±1.14) had a similar effectiveness to Hydrochlorothiazide (1.52±1.23). In the gentamicin-induced nephritis model, ARUR-10 reduced serum creatinine by 42.8%, uric acid by 14.9%, and urea by 32% at doses of 240 mg/kg, compared to the control group that produced the pathological model. It had a protective effect on the kidneys by preserving the tube structure; cell death and cell necrosis were not observed.

Keywords: 2.2-diphenyl-1-picrylhydrazyl; flavonoid; nephroprotective activity; phenolic compounds; traditional ARUR-10 prescription

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