Green Synthesis of Silver Nanoparticles using Cardiospermum Halicacabum Leaf Extract and its Effect on Human Colon Carcinoma Cells

Venkatesan Kotteeswaran^*, Shruthi Ponsreeram, Aritra Mukherjee, Anirudh Sadagopanand Naveen Kumar Anbalagan

Department of Biotechnology, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India,

Corresponding Author E-mail: venkatek@srmist.edu.in

Abstract: Nanobiotechnology is an evolving domain of scientific exploration connected with synthesis and mechanism of nanoparticles in biological systems. Silver nanoparticles have gained utmost popularity because of its characteristics like thermal conductivity, chemical stability, and high catalytic activities. It also has various advantages like being a drug carrier, transmembrane deliver and potential for controlled intracellular drug-delivery. The current study deals with fabrication of nanoparticles which are formed from biologically reduced silver, followed by its characterization. In addition, anticancer activity was investigated using in-vitro cell model. The synthesis was confirmed from the change in color to brown from golden yellow and also from absorbance peak obtained at 430nm in UV-Vis spectrophotometry. The obtained nanoparticle had an average size of about 150.1 nm and its FTIR peaks suggested the presence of flavonoids and polyphenols. XRD analysis exhibited peaks in the 2θ range of 227.72^o, 32.29^o, 38.76^o, 43.17^o, 54.47^o, 64.04^o and 77.75^o, is confirming its crystallographic nature. Further, anticancer activity of silver nanoparticles was tested through cell proliferation assays, Lactate Dehydrogenase assays, and apoptosis assay using Hoechst/PI staining, wherein there is clear reduction in cell’s proliferation, viability, and LDH release, followed by increased dead cells as a result of treatment.

Keywords: Anticancer Activity; Apoptosis; Cardiospermum halicacabum; Cell based assay; Green synthesis; Silver nanoparticles  

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