Comparison Of Anticancer Activity Between Thymoquinone And Tamoxifen , Thymoquinone + Tamoxifen On Mcf -7 Cell Line Of Human Breast Cancer –An Invitro Study.

Neevedha K¹_,  Anitha E¹, Thulasi Gokul² , Isswariya Anandan¹ and S Priestly Vivekkumar¹

¹Department Of Pharmacology, Panimalar Medical College  Hospital and Research Institute, Poonamallee, Chennai, tamil nadu India.

²Department of Pharmacology, Government Medical College, Ongole, Andhra Pradesh, India.

Corresponding Author E-mail: neevedhakannan@gmail.com

Abstract: This study aims at evaluating the anticancer effect on the MCF-7 (Michigan Cancer Foundation-7) cell line of human breast cancer using Thymoquinone and Tamoxifen alone as well as in combination therapy by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) Test. NCCS in Pune provided the MCF-7 cell line. The cells were kept at 37°C in a humidified medium of 50µg/ml CO₂ in Minimal Essential Medium added with 10percent FBS (Foetal Bovine Serum), streptomycin (100µg/ml), as well as penicillin (100U/ml). MTT-(3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl tetrazolium bromide) test was conducted on MCF-7 cell line for Thymoquinone and Tamoxifen as sole and combination therapy. Measurements were performed using UV (Ultra-Violet)-spectrophotometer at 570-nanometre absorbance and the content needed for a 50 percent inhibitory concentration (IC50) was calculated and evaluated graphically. IC 50 of Thymoquinone on MCF 7 was found to be at 31.2 µg/ml and Tamoxifen was at 62.5 µg/ml were as in combination therapy the IC 50 was found to be at 7.8 µg/ml. There is a remarkable reduction in concentration to achieve IC 50 percentage in combination therapy with a comparison with individual therapy. Therefore, the combination therapy of Thymoquinone and Tamoxifen on the MCF-7 cell line is more efficacious when compared to individual treatment on cell viability inhibition.

Keywords: Inhibitory Concentration; Michigan Cancer Foundation-7, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; Tamoxifen; Thymoquinone

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