Omics-based Analysis of Bhadradarvadi Kashayam in Managing Rheumatoid Arthritis via CXCL8-CXCR1/2 axis, MAPK and NF-κB Signaling Pathways - A Network Pharmacology Approach
Mohamed Thoufic Ali A M
and Vino Sundararajan*Integrative Multiomics Laboratory, Department of Bio Sciences, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
Corresponding Author E-mail: svino@vit.ac.in
Abstract: With the advances in the field of medicine there is an increase in the geriatric population and rheumatoid arthritis is one of the common diseases that affect this cohort. The modern medicines that are used for the treatment of rheumatoid arthritis provide a symptom-based treatment and there are studies showing severe side effects for some of the medicines being used. But there are shreds of evidence in traditional medical texts for the treatment of rheumatoid arthritis which gives an increased therapeutic coverage with less to no side effects. Bhadradarvadi kashayam (concoction) is one of the most commonly preferred and prescribed Ayurvedic medicine for managing the disease. In this study, we are investigating the mode of action of this kashayam by employing a network pharmacology-based framework which included the analysis of the cross-talks between the active ingredients of the kashayam and major molecules involved in the disease, the transcription factors and various pathways in which they are involved. Based on the systems pharmacology approach, 57 active compounds and a total of 377 potential targets with their interacting partners, and the targets associated with comorbidities were identified. The PPI network was analyzed to understand the topological index for screening the hub proteins such as MAPK1, MAPK14, FYN and CXCL8, which were found to be enriched in various signaling pathways. Furthermore, molecular docking analysis validated the strong physical interaction between the hub proteins and the corresponding active compounds from BDK. Overall, the study sheds light on the pharmacological mechanism of Bhadradarvadi kashayam against Rheumatoid Arthritis and also highlights that there are traditional herbal remedies imparted by the Ayurveda system of medicine which has the least side effects compared to modern medicines.
Keywords: Auto-immune diseases; Bhadradarvadi; Inflammation; Rheumatoid arthritis; Signaling pathways; Systems pharmacology Back to TOC