Modulation of Immune Response from FiberCreme-VCO Based Supplementation in Immunosuppressed Rats
Devyani Diah Wulansari1*
, Dwi Winarni2, Lanny Sapei3, Marisca Evalina Gondokesumo4, Raden Joko Kuncoroningrat Susilo5, Rochmad Indrawanto6, Rhaesfaty Galih Putri6, Suhailah Hayaza5 and Rizqiyatul Fithriyah11Department of Clinical and Community Pharmacy, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut, Surabaya 60293, East Java, Indonesia
2Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia
3Department of Chemical Engineering, Faculty of Engineering, University of Surabaya, Raya Kalirungkut, Surabaya 60293, East Java, Indonesia
4Department of Biology Pharmacy, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut, Surabaya, East Java, Indonesia
5Unit of Nanotechnology Engineering, Department of Engineering, Faculty of Advance Technology and Multidiscipline, Universitas Airlangga, Surabaya, Indonesia
6PT Lautan Natural Krimerindo, Mojokerto, Indonesia
Corresponding Author E-mail: devyanidiahwulansari@staff.ubaya.ac.id
Abstract: Immunomodulators are substances that have the ability to influence the human immune system. FiberCreme is a commercial non-dairy food high in oligosaccharides, which are difficult to digest. Combination of FiberCreme and VCO (FC-VCO) considered to possess high value of bioavailability in body. This study was aimed to determine immunomodulation effect of FiberCreme-VCO against immunosuppressed rats. This study used male rat (Rattus novergicus) which was induced by doxorubicin twice a week for seven days. Treatment by FC-VCO was then administered orally for 14 days. Whole blood and spleen were collected and analyzed based on immunomodulator parameters such as spleen weight, body weight, IL-6, TNF-α, and INF-γ levels, the CD4+/CD8+ ratio and the percentage of FOXP3. Statistical analysis was determined by GraphPad Prism software (version 9: San Diego, CA, USA). Regardless of dosage, FC-VCO did not improve body weight or lymphatic weight appreciably. In contrast to the negative group, FC-VCO supplementation at a dose of 6 mg/kgBW was able to raise CD4+ levels, and this difference was statistically significant (p<0.05). IFN-γ levels were also increased by FC-VCO at a dose of 9 mg/kgBW, and these differences were statistically significant (p <0.05) when compared to the negative group. Since FC-VCO affects the roles and responsibilities of CD4+ and IFN-γ in immunosuppressive situations, it can strengthen the immune system.
Keywords: Doxorubicin; FiberCreme; Healthy Life; Immunomodulator; Virgin Coconut Oil Back to TOC