Preclinical Evaluation of Nerolidol’s Hepatoprotective and Nephroprotective Potential

Pavan Udavant^1*, Gayatri Kanade¹, Shubham Khairnar¹, Rahul Sable¹, Neelam Dashputre¹, Anjali Tajanpure¹, Dinesh Rishipathak², Santosh Chhajed², Musab Tanzeel¹, Simona D’Souza¹ and Naveed Ahmad¹

¹Department of Pharmacology, MET’s Institute of Pharmacy, Bhujbal Knowledge, City, Adgaon, Nashik Maharashtra, India.

²Department of Pharmaceutical Chemistry, MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik Maharashtra, India.

Corresponding Author E-mail: pavanudavant@gmail.com

Abstract: Background: Due to the potential negative effects of artificial food additives on health and the recent surge in consumer awareness of the issue, natural goods are becoming more and more popular in diets. Objective: The main objective of the current study is the evaluation of the protective effect and antioxidant role of Nerolidol against alloxan-induced oxidative stress, hepatotoxicity, and nephrotoxicity.  Method: The present experiment was designed as Group I (control), Group II (Alloxan monohydrate, 120 mg/kg i.p), Group III (Ascorbic acid 250 mg/kg p.o), Group IV (Nerolidol 100 mg/kg p.o), Group V (Nerolidol 200 mg/kg p.o), Group VI (Nerolidol 300 mg/kg p.o). Alloxan was given to all groups excluding control group in order to induce hepatorenal toxicity. The groups III, IV, V and VI received the Standard Ascorbic acid and Nerolidol after 72 hrs. of alloxan administration for consecutive 14 days. The protective roles and antioxidant activity of Nerolidol against Alloxan induced oxidative stress and hepatorenal toxicity were evaluated by histopathological changes, measuring hepatic and renal damage biomarkers, antioxidant enzyme levels and malondialdehyde (MDA) parameters in the liver and kidney tissues of rats. Result and Conclusion: The biochemical analysis showed a decrease in serum AST, ALT, ALP, and LDH enzymes, total protein, creatinine, bilirubin, and urea in group III and test groups compared to that of group II. Nerolidol also restored the Alloxan-induced MDA and antioxidant enzyme level to control. Hepatorenal protection of Nerolidol was confirmed by almost normal histological findings in test groups.

Keywords: Antioxidant; Biomarkers; Hepatotoxicity; Nephrotoxicity; Oxidative Stress

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