Effect of Organic Additives on Activity of Urease
C .S. Felice*, Purbaj Pant, Bhim Gurung, Akshaya Neupane and Bijay DhungelDepartment of Biotechnology, Koneru lakshmaiah College of Engineering Vaddeswaram- 522502, Guntur Dist, Andhra Pradesh (India).
Corresponding Author E- mail: Shechinahfelice@yahoo.co.inAbstract: Urea has become the most used nitrogen fertilizer in the world, accounting for approximately 40% of the totally nitrogen supply. Its market share is increasing since it is the least expensive form of solid nitrogen fertilizer and its high nutrient content (46%N). Much of the nitrogen in fertilizer comes from urea, which bacteria degrade into ammonia and CO2 using urease.Its efficiency is however decreased by losses of nitrogen through ammonia volatilization by urease enzyme catalyzing it. In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and several higher plants. Urease is significant in the history of Enzymology as the first enzyme to be purified and crystallized. The present study was undertaken to study the isolation of the enzyme and the effect of various activators and inhibitors on the activity of the enzyme
Keywords: Urea; urease; Estimation; Modulators; Inhibition; spectrophotometry Back to TOC