Evaluation of the Cerebroprotective Properties of Ademol-gel in the Analysis of Specific Indicators in the Open Field Test
Sviatoslav Semenenko1
, Igor Semenenko2, Bogdan Burlaka3, Iryna Samura4* , Nina Bukhtiyarova5 , Viktor Ryzhenko6 and Olga Khromylova7
1 Department of Clinical Pharmacy and Clinical Pharmacology, National Pirogov Memorial Medical University, Ukraine.
2 Department of Therapeutic Disciplines and Family Medicine, National Pirogov Memorial Medical University, Vinnitsa, Ukraine.
3 Department of Medicines Technology, Zaporizhzhia State Medical University, Ukraine.
4Department of Pharmacology and Medical Formulation with the Course of Normal Physiology, Zaporizhzhia State Medical University. Ukraine.
5Department of Clinical Laboratory Diagnostics, Zaporizhzhia State Medical University, Ukraine.
6Department of Medical and Pharmaceutical Informatics and Advanced Technologies, Zaporizhzhia State Medical University, Ukraine.
7Department of Pharmaceutical, Organic and Bioorganic Chemistry, Zaporizhzhia State Medical University, Ukraine.
Corresponding Author E-mail: irinasamura77@gmail.com
Abstract: Experimental subarachnoid hemorrhage (SAH) modeling in rats led to a significant, by 3 times, decrease in the total activity of animals, a decrease in the distance traveled by 2 times, a significant increase in inactivity when moving from the dark field to the illuminated part by 2 times, indicating a decrease in locomotor and search activity. SAH did not affect the number of unsupported rearing, but led to an increase in supported rearing against the wall, and a decrease in the number of short grooming acts against the background of an unchanged amount of long grooming. This behavior also testified to increased anxiety, excitability, irritability of animals, discomfort, and depression. The intranasal introduction of Ademol-gel at a dose of 2 mg/kg (a developed original intranasal form) to animals with SAH contributed to the positive emotional status and behavior of rats in the open field test, as evidenced by a decrease in anxiety, aggressiveness, and depressive behavior against the background of increased comfort and empathy in animals. Against the background of the intranasal introduction of Ademol-gel, the processes of restoring general motor and exploratory activity in animals after SAH were also accelerated. Nimodipine (30 μg/kg) was inferior to Ademol in terms of the degree of influence on the studied parameters of animal behavior. The results are an experimental justification for further study of a new dosage form – Ademol intranasal gel.
Keywords: Ademol, Cognitive Deficit, Subarachnoid Hemorrhage Back to TOC