Reverse Phase High Performance Liquid Chromatographic Determination of Ezetimibe in Bulk and Pharmaceutical Formulations
Ch. Narasimharaju BH1, G. DevalaRao2 and Rajendran Rukmani3¹A.S.N.Pharmacy College, Burripalem Road, Tenali - 522 201 (Inda).
²KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada - 520 010 (India).
³Jaya College of Pharmacy, Thiruninravur - 602 024 (India).
Corresponding Author E-mail: bhchnraju@yahoo.com
Abstract: This paper describes about validated Reverse phase High Performance Liquid Chromatographic method for determination of Ezetimibe in bulk and pharmaceutical formulations has been developed. Chromatographic separation was carried out using a mobile phase of Potassium dihydrogen phosphate buffer (containing triethylamine) and acetonitrile (55:45v/v) on a C18 –ODS –Hypersil (5 µ, 250×4.6 mm) column in isocratic mode at a flow rate of 1.5ml/minute with UV detection at 234nm. Atorvastatin calcium was used as internal standard. The chromatographic method was linear over the concentration range of 50-250ìg/ml. The Limit of Detection and Limit of Quantitation were 0.828 and 2.5 µg/ml respectively. The mean percentage purity and recovery were found to be 101.22% and 100.36% respectively. Suitable method was developed to determine Ezetimibe in bulk and pharmaceutical formulations.
Keywords: RP -HPLC; Ezetimibe; Tablets Back to TOC