Compendium of P. Scrobiculatum: Phytochemical, Physicochemical, Antioxidant, Antiulcer, and Spectroscopic Analysis
Manisha Bhatti1
, Jitender Singh2 and Divya Dhawal Bhandari 3*1University Institute of Pharma Sciences, Chandigarh University, Gharuan, Mohali (Punjab), India
2Institute of Pharmaceutical Sciences, IET Bhaddal Technical Campus, Bhaddal, District Rupnagar, Punjab India
Corresponding Author E-mail: nainagumber@gmail.com
Abstract: Background: Paspalum Scrobiculatum (Kodomillet) plant is found in various regions of India and possesses several pharmacological activities such as nutritive, anti-fungal, anti-diabetic, etc. The researchers are more focused these days on exploring other potential activities of this plant and the constituents present. Methods: Present study emphasized preliminary analysis such as macroscopic study, phytochemical analysis, Physicochemical investigation, determination of ash content, (Total flavonoid content), antioxidant property and characterization of extracted components with different spectral techniques and antiulcer potential. later, this study will be helpful in the determination of its medicinal importance in Pharmacy. Results: In macroscopic analysis, it was studied that paspalum scrobiculatum seeds are light brown to dark brown colored, round to oval shaped grain having a size approximately 4mm. Further, the seeds were analyzed for determination of the extractive values and found to be 2.82 % w/w, 0.79 %w/w, 4.26 % w/w 2.40 % w/w in pet. ether, chloroform, ethanol, and water extracts respectively, and extractive value was found highest in ethanol extract. Furthermore, phytochemical analysis of all the different extracts revealed the presence of phenolic components, flavonoids amino acids, protein, and carbohydrates in ethanolic extract, whereas in aqueous extract there was the presence of saponins and carbohydrates. Then, spectral characterization analysis was done with Infrared spectroscopy, Nuclear magnetic resonance, and Mass spectroscopy techniques and revealed the presence of different active metabolites in the ethanolic extract which may be considered for numerous pharmacological properties, thus further processed for anti-ulcer potential. The ethanolic extract has given promising results as an antiulcer agent against the standard drug omeprazole. Conclusion: The present research concluded from various parameters such as macroscopic evaluation, successive extraction, physicochemical investigation, phytochemical investigation, and spectral analysis that ethanolic extract of plant P. Scrobiculatum contains the maximum amount of secondary metabolites and was further investigated for anti-ulcer potential at a dose of 250 mg/kg and 500 mg/kg and the mean ulcer index was found to be 3.39±0.10 and 2.78±0.10 respectively against the standard drug omeprazole.
Keywords: Antioxidant Activity; Antiulcer potential; Paspalum Scrobiculatum; Physicochemical Properties; Phytochemical Profile; Spectral Analysis; TPC; TFC Back to TOC