Neuroprotective Activity of Ethanolic Extract of Red Ginger Containing 6-Shogaol on Scopolamine-Induced Memory Impairment in Alzheimer's Mice
Fifteen Aprila Fajrin1,2*
, Dina Permatasari2, Devira Asdar2 and Ika Puspita Dewi1,21Department of Clinical and Community Pharmacy, Faculty of Pharmacy, Universitas Jember, East Java 68121, Indonesia
2Preclinical Pharmacology Research Group Faculty of Pharmacy, Universitas Jember, East Java 68121, Indonesia.
Corresponding Author E-mail: fifteen.farmasi@unej.ac.id
Abstract: Red ginger contains 6-shogaol, which has a neuroprotective effect and is crucial in several brain diseases, including Alzheimer’s. This study investigated the neuroprotective effect of red ginger extract (RGE) containing 6-shogaol on scopolamine-induced memory loss in mice. Male Balb/C mice (n = 30; 6–7 weeks old) were divided into six groups: normal, Alzheimer, drug control (donepezil), and RGE (100, 200, and 400 mg/kg BW). In the acute experiment, the mice were treated 60 minutes before the test, followed by 1 mg/kg of scopolamine 30 minutes later. Thirty minutes later, the mice were placed individually in a Y-maze to observe spontaneous alteration activity (SAA). In the chronic experiment, the mice were treated once daily for seven days. On days 8–14, the mice were administered scopolamine and treatment. SAA was observed every 3–4 days. On day 15, malondialdehyde (MDA) and acetylcholinesterase (AChE) levels in the serum and brain were determined. RGE treatment 400 mg/kg BW, containing 1.664 µg of 6-shogaol per 100 mg of ethanol RGE, reduced memory loss better than the other two doses. RGE successfully decreased MDA and increased AChE in the serum and brain. RGE also showed effectively to improve memory in Alzheimer’s disease.
Keywords: Alzheimer; memory loss; neuroprotective; red ginger extract; 6-shogaol Back to TOC