Evaluation Cytotoxicity Effects of Centaurea Cineraria Extracts Against some of Cancer Cell Lines
Sumayah Sami Hashim1
, Zainab Farqad Mahmood1, Safa M. Abdulateef 2*, and Batol Imran Dheeb 31University of baghdad, College of Science, biotechnology department, Iraq.
2Dijlah University college, Medical laboratory techniques, Baghdad, Iraq.
3Samaraa University, College of applied science, pathological analysis, Iraq.
Corresponding Author E-mail: batoolomran@yahoo.com
Abstract: This research included the detection and determination of flavonoids from Centaurea Cineraria extract collected from public gardens using high-performance liquid chromatography technique. The compounds (Cirsilineol, Jaceosidin, Melitensin) were found, their concentrations were measured and found (36254,63719,89035 µg/ml), and qualitative chemical detections were performed. Study of the effect of the methanolic extract of Centaurea Cineraria containing the flavonoids mentioned above. The extraction process was carried out using an ordinary organic solvent ethanol and using a rotary evaporator device. The flavonoids present in the extract were detected, using standard flavonoids (Cirsilineol, Jaceosidin, Melitensin) and study the effect of the extacts on some cancer cell line also study on utilizing stable free extreme compound 1, 1-Diphenyl-2-Picryl-hydrazil (DPPH). Results uncovered that the more expanding in centralization of the concentrate the seriously rummaging level of the free extremists. The fixations that were utilized gone from36.12 to 2000µg/ml, considered the Methanol concentrate of Centaurea Cineraria essentially showed high anticancer activity agent movement (96% at 2000µg/ml) connected with hexane extricates which were 94.61% at 2000µg/ml. This work additionally planned to concentrate on the cytotoxic and anticancer impacts of Centaurea Cineraria extricates The predominant decrease in attainable count cell was 500/ml in various cell lines, which was associated to methanolic isolates and appeared to cause cell loss in the cell lines L20B, PC-3, and HCT116 separately.
Keywords: Centaurea Cineraria; Cancer cell lines; Cytotoxicity; DPPH; HCT116; L20B; PC-3 Back to TOC