Partial Purification and Optimization of Alkaline Protease from Bacterial ncim 2724 Species by Solid State Fermentation.
V. Praveen Kumar¹*, K. Shrinivasulu² and P. Kalpana³¹Lecturer, Department of Biotechnology, K.L College of Engineering, Green Fields, Vaddeswaram, Guntur District (India).
²Lecturer, Department of Biotechnology, K.L College of Engineering, Green Fields, Vaddeswaram, Guntur District (India).
³Department of Biotechnology, K.L College of Engineering, Green Fields, Vaddeswaram, Guntur District (India). Corresponding Author: vemuripraveen_bt@klce.ac.in
Abstract: Production of alkaline protease employing the laboratory isolate, Bacillus sp. under solid state fermentation (SSF) was optimized. The effect of wheat bran was examined. The appropriate Incubation time, Temperature, pH, Moisture content, Inoculums size, Surfactant and mutagen effect were determined. Maximum yields of enzyme were achieved by employing wheat bran as substrates in 0.1 M carbonate/bicarbonate buffer at pH 10 with 1:1.5 moisture content at 48 hrs. Inoculums size and buffer solution level were found to be 30% for wheat bran. The increased growth of bacterium of mutagenic activity is seen with acrylamide solution.
Keywords: Alkaline protease; SSF; NCIM Back to TOC