Melittin-Induced Cell Death Through p53 and 8-OHdG in Breast Cell Cancer MCF-7
Makkasau Plasay1,2*
, Rosdiana Natzir3, Muhammad Husni Cangara4,5, Marhaen Hardjo3, Syahrijuita3 and Gita Vita Soraya31Master of Biomedical Study Program, Post Graduate School, Hasanuddin University, Makassar, South Sulawesi, Indonesia
2Panakkukang College School of Health Sciences, Makassar, South Sulawesi, Indonesia
3Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia
4Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia
5Installation of Anatomical Pathology, Hasanuddin University Hospital, Makassar, South Sulawesi, Indonesia
Corresponding Author E-mail: makkasau_mkes@yahoo.co.id
Abstract: Melittin, one of the cytolytic peptides derived from bee venom, is a broad-spectrum efficacy candidate as an antibacterial, antifungal, and antitumor agent. This study demonstrates the cytotoxic effect of melittin isolated from Apis mellifera through the induction of p53 and 8-OHdG. The antiproliferative effect was evaluated against breast cell cancer MCF-7 via MTT assay, while the molecular mechanism of melittin on MCF-7 was assayed by p53 and 8-OHdG ELISA. With an IC50 value of 5.86 µg/mL ((very toxic)), the cytotoxic impact inhibits MCF-7 cell proliferation in a dose-dependent manner. Significant (p < 0.05) elevations in the level of p53 and 8-OHdG were evident in the IC50-treated cells compared to control. In conclusion, melittin may have considerable potential as a novel natural product-based for breast cancer.
Keywords: Cytotoxic; Melittin; MCF-7; 8-OHdG; p53 Back to TOC