Effects of Amnion Lyophilization Sterile Radiation on Vascular Endothelial Growth Factor and Cluster of Differentiation 34 in Nonunion Fractures

Ahmad Fauzi^1,3*, Alvarino², Yanwirasti² and Roni Eka Sahputra²

¹Student of Doctoral Program in Biomedical Science, Universitas Andalas, Padang, Indonesia.

²Faculty of Medicine, Universitas Andalas, Padang, Indonesia.

³Faculty of Medicine, Universitas Lampung, Bandar Lampung, Indonesia.

Corresponding Author E-mail: ahmadfauzi_dr@yahoo.co.id

Abstract: One of the causes of nonunion is inadequate vascularization due to severe injury mechanisms that cause defective bone healing factors. Therefore, in non-union fractures, a trigger is needed for the growth factors to work properly. This study aimed to determine the effects of ALSR on  VEGF and CD-34 in non-union fractures. This study used an experimental post-test only control group design that involved white rats of the Sprague-Dawley strain. The VEGF expression was assessed by anti-VEGF staining, which was done by cutting paraffin blocks into a thickness of 5 μm, then the blocks were deparaffinized in xylol and rehydrated with alcohol. The results of the analysis are displayed with their mean ranks between control groups and treatment groups. It was found that CD-34 expression was high in the ALSR group. This shows the occurrence of angiogenesis and the regeneration in the case of non-union fractures in this study.

Keywords: Amnion Lyophilization Sterile Radiation; Cluster of Differentiation 34; Nonunion fractures; Vascular Endothelial Growth Factor

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