Effect of Burdock Root Oil on Oxidative Stress Induced by Isolated and Combined Use of Gamma Radiation and Hexavalent Chromium

Marat Iztleuov¹, Yerbolat Iztleuov^1*, Samat Saparbayev¹, Assem Temirbayeva¹, Raushan Medeuova¹, Zarina Aleuova², Irina Ismailova¹, Nauryzbai Imanbayev¹

¹West Kazakhstan Marat Ospanov Medical University. Aktobe, Kazakhstan

²West Kazakhstan M. Utemisov University.

Corresponding Author E-mail: ermar80@mail.ru

Abstract: They studied the effect of the herbal medicine "Burdock root oil" on oxidative damage to liver, kidney and blood tissues. The experiment was performed on 50 non-linear white male rats weighing 180-220 g, divided into 5 groups. The first group - control; animals of groups II and III were subjected to fractional gamma irradiation for five days (0.6 G/day; dose rate 1 Gr/min (⁶⁰Co)). The total dose was 3 Gr. Animals of the fourth and fifth groups were exposed to a combination of gradiation (as in groups I and III) and potassium dichromate (Cr⁺⁶). Potassium dichromate was administered intraperitoneally daily at a dose of 2.8 mg/kg of body weight (0.1^LD₅₀) for 5 days (0.5^LD₅₀). Rats of groups 3 and 5 received Burdock root oil at a dose of 2.5 ml/kg of body weight intragastrically for 14 days prior to the experimental exposure. Fractional exposure, combined exposure g-radiation, gamma radiation and Cr⁺⁶ led to an increase in malondialdehyde and diene conjugates in blood plasma, liver and kidney tissues. Under g-irradiation, the activity of superoxide dismutase enzymes (SOD) and catalase (CAT) in red blood cells compensation increased significantly against the background of a decrease in the level of SH-groups in blood plasma. In liver and kidney tissues, all studied enzymes and reduced glutathione (GSH) levels decreased. Under the conditions of combined exposure g-radiation and potassium dichromate - all the studied indicators of antioxidant protection decreased. The introduction of Burdock root oil before isolated and combined exposure provided significant antioxidant protection in the studied tissues. Conclusion: it can be assumed, that the "Burdock root oil" it is a potential drug that can be used as a radiation protector, in conditions of combined influence of a physical and chemical agent-a detoxifier. In our opinion, the antioxidant potential of the herbal medicine justifies the continuation of further research in clinical practice.

Keywords: Antioxidant Potential; Combined Effect of Gamma Irradiation and Cr; Fractional Gamma Irradiation; Herbal Medicine "Burdock Root Oil"; Liver, Kidney, Blood; Oxidative Damage

[ HTML Full Text]

Back to TOC