The changes of HIF-1α and ICAM-1 expression after Miana (Coleus scutellariodes [L]) treatment in Balb/c mice with Mycobacterium tuberculosis infection

Rosamarlina^1,2, Mochammad Hatta^2,3,, Irawaty Djaharuddin⁴ , Ilhamjaya Patellongi⁵, Agus Dwi Susanto⁶, Andi Asadul Islam⁷, Muhammad Nasrum Massi³, Agussalim Bukhari⁸, Arif Santoso⁴, Nur Ahmad Tabri⁴, Farida Murtiani¹, Ade Rifka Junita^2,3, Ahmad Syukri Saleh^2,3, Ressy Dwiyanti⁹ and Sesilia Rante Pakadang¹⁰

¹Sulianti Saroso Infectious Diseases Hospital, Jakarta, Indonesia

²Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

³Molecular Biology and Immunology Laboratory, Faculty of Medicine Hasanuddin University, Makassar, Indonesia.

⁴Department of Pulmonology and Respiratory Medicine, Faculty of Medicine Hasanuddin University, Makassar, Indonesia.

⁵Department of Physiology, Faculty of Medicine Hasanuddin University, Makassar, Indonesia.

⁶Department of Pulmonology and Respiratory Medicine, Persahabatan Hospital, Jakarta, Indonesia.

⁷Department of Neurosurgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

⁸Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

⁹Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia

¹⁰Department of Pharmacy, Health Polytechnic of Makassar, Indonesia

Corresponding Author E-mail: hattaram@yahoo.com

Abstract: Background: Increasing resistance to TB drugs raises the challenge of TB eradication. Miana leaves is Indonesian traditional herbal medicine, have antimicrobial, anti-inflammatory, and immunoregulatory action. Not much is known about the effect of Miana on HIF-1α and ICAM-1, the immunoregulators of infection and inflammation. This study aims to elucidate the effect of Miana on HIF-1α and ICAM-1 in M. tuberculosis (Mtb) infected mice. Materials and Methods: This experimental study used Mtb infected Balb/c mice were divided into 4 groups; group 1 is placebo, group 2 is treated with Rifampicin as Anti TB drug, group 3 is treated with Miana, and group 4 is treated with Miana + Anti TB drug. HIF-1α and ICAM-1 serum levels were analyzed using ELISA. Results: There is a significant difference of mean HIF-1α (p= 0.00, F = 114.21) and ICAM-1 (p= 0.00, F = 113.11) between the four groups after treatment. HIF-1α level is significantly lower in anti TB treatment, Miana, and Miana + anti TB treatment compared to placebo (mean difference (MD) 35,764.67, p=0.00; 29,230.98, p=0.000; 38,489.62, p=0.00, respectively). Furthermore, ICAM-1 level is significantly lower in anti TB treatment, Miana, and Miana + anti TB treatment compared to placebo (MD 95,449.68, p=0.00; 79,509.69, p=0.00; 108,672.83, p=0.00, respectively). Conclusion: HIF-1α and ICAM-1 expression was reduced after Miana administration. Miana can be a potential complement to anti-TB treatment but cannot replace rifampicin as anti-TB drugs.

Keywords: Coleus scutellariodes; HIF-1α; ICAM-1; Miana leaf; M. tuberculosis; Rifampicin

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