Glucosinolates with Their Hydrolysis Products from Two Cruciferous Plants with Study of Antidiabetic Activity Based on Molecular Docking

Suliman A. Alderhami¹ , Khaled A. Abdelshafeek^1,2,*, Walid E. Abdallah² , Ahmed A. Elhenawy^1,3  and  AbdulAziz Ali Alomari¹

¹Department of chemistry, faculty of sciences and arts, Mukhwah , Albaha university, Albaha, Saudi Arabia

²Department chemistry of medicinal plants, Pharmaceutical Industries Div., National Research Centre, 33 El Buhouth St.(Former El Tahrir St.), 12622-Dokki, Giza, Egypt.

³Chemistry Department, Faculty of Science, Al-Azhar University (Boys Branch),Nasr City, Cairo, Egypt.

Corresponding Author E-mail: khabdelhady@gmail.com

Abstract: Glucosinolates (Gls) are natural bioactive compounds that form metabolites called isothiocyanates (ITC) which have various therapeutic effects. This study aimed to isolate the glucosinolates of Carrichtera annua L.(DC) (CA) and Farsetia aegyptia Turra (FA) belonging to the Crucifereae family. Total Gls were isolated from the aqueous methanolic extract of the plants and further purified using an acidic aluminum oxide column. Some of the obtained Gls were identified via spectroscopic methods (UV, NMR, and MS) and the rest were hydrolyzed by myrosinase to the corresponding isothiocyanates (ITC) for identification by GC/MS. Only one Gls was identified in CA as 4-methylthio-3-butenyl Gls (MTBG) in addition to 6-methyl sulfonylhexyl isothiocyanates (ITC), while 6-methyl sulfonyl-6-hydroxy hexyl ITC, 4-pentenyl ITC, 3-methylthio propyl ITC, 5-hydroxy pentyl ITC and 4-methylsulphinyl butyl ITC were identified in FA. The Gls demonstrated high binding activity to α-glucosidase and amylase, good pharmacokinetic characteristics, and exerted no carcinogenetic effects.

Keywords: Crucifereae; Carrichtera annua; Docking; Farsetia aegyptia; Glucosinolates and Isothiocyanates; GC/MS

[ HTML Full Text]

Back to TOC