UV Spectrophotometric Method for Quantification of Rivastigmine Tartrate in Simulated Nasal Fluid: Development and Validation
Deepshi Arora1, 2
, Manish Kumar1 , Shailendra Bhatt3, Yugam Taneja4, Abhishek Tiwari5 and Varsha Tiwari51M.M. College of Pharmacy, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana-133207, India
2Guru Gobind Singh College of Pharmacy, Yamuna Nagar, Haryana- 135001 India
3Department of Pharmaceutics, Department of Pharmaceutics, School of Medical and Allied Sciences, G.D. Goenka University, Gurugram, Haryana-122103 India.
4Zeon Lifesciences Pvt.Ltd, Paonta Sahib, Himachal Pradesh-173025 India
5Department of Pharmacy, Devsthali Vidyapeeth College of Pharmacy, Rudrapur, U. S. Nagar (Uttarakhand)-263148 India
Corresponding Author E-mail: manish_singh17@rediffmail.com
Abstract: Background: Rivastigmine Tartrate belongs to the class of cholinesterase inhibitors in Anti-alzheimer’s disease with optimum therapeutic efficacy. Till now no validated method of its quantification has been reported in simulated nasal fluid. Objective: The current research investigation aims to develop a rapid, simple, and reliable UV spectrophotometric method for the quantitative determination of the pure form of Rivastigmine Tartrate in SNF. Method: A suitable method was developed by using double beam UV spectrophotometer and selection of a suitable solvent system for estimation of Rivastigmine Tartrate at absorbance maxima 263nm in SNF. The method was validated for various parameters like including accuracy, linearity and precision as per the International Conference on Harmonization guidelines. Results: The method developed by selecting simulated nasal fluid as the solvent system satisfied the optimum condition of the good quality peak at the selected wavelength. The results proposed the developed method for Rivastigmine Tartrate quantification in the simulated nasal fluid to be linear in the working concentration range of 5-60 µg/ml with a co-relation coefficient of 0.998. The % accuracy was found to be 99.8 -100.57. The % RSD values were < 2 while LOD & LOQ values were detected to be 0.316 and 1.053 respectively. Conclusion: The stated method was analyzed to be rapid, accurate, reliable, and precise. Further, it can be used in checking the quality control parameters of the Rivastigmine Tartrate in routine analysis.
Keywords: Inter-Day; Intra-Day; Rivastigmine; Spectrophotometer; Simulated Nasal Fluid Back to TOC