Antidiarrheal Activity of Four Different Species of Litsea Available in Bangladesh
Israt Jahan Bulbul1,2
, Md. Ekhtiar Uddin1, Nusratun Nahar1, Md. Ruhul Kuddus2, Mohammad Rashedul Haque2, Mohammad Abdur Rashid2*1Department of Pharmacy, Southeast University, Banani, Dhaka-1213, Bangladesh.
2Phytochemial Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh.
Corresponding author E-mail: r.pchem@yahoo.com
Abstract: The objective of the present study includes the evaluation of the antidiarrheal properties of the methanol extracts of Litsea deccanensis Gamble (MELD) bark, Litsealancifolia (Roxb.) Hook. f. MELL),Litseaglutinosa Gamble (MELG) and Litsea monopetala Roxb. (MELM) leavesin Swiss albino mice. The antidiarrheal activity was evaluated by measuring percentage inhibition of diarrheal feces, total fecal output, gastrointestinal motility and by using peristaltic indices. Castor oil was used to induce diarrhea in the experimental animal. The experiments were carried out by using three different doses (100, 200, and 400 mg/kg body weight) of these four plant extracts. The number of wet feces and total weight of the feces were significantly (p < 0.05) and dose-dependently reduced by all the plant extracts and this effect was comparable with standard drug. MELD, MELL, MELG and MELM extracts at dose of 400 mg/kg body weight demonstrated diarrheal inhibition by 43.55%, 45.16%, 32.26% and 41.94%, respectively while it was 98.39% for the standard loperamide. Percentage (%) of fecal output for MELD, MELL, MELG and MELM extracts at the dose of 400 mg/kg were 40.14%, 62.27%, 64.06%, 46.26%, respectively.The gastrointestinal motility induced by castor oil was also reduced noticeably (p < 0.05) by all the plant extracts with the increasing doses. The percentage inhibition of gastrointestinal motility at the dose of 400 mg/kg were 26.26%, 33.22%, 32.36% and 22.52% for the MELD, MELL, MELG and MELM extracts respectively, while it was 27.56% for loperamide. In most cases, all the plant extracts can reduce the peristaltic indices which were comparable to control. The obtained results from this study revealed that the methanol extracts of four different species of Litsea found in Bangladesh may have antidiarrheal potential. It also provides the basis for the traditional use of these plants to treat diarrhea.
Keywords: Antidiarrheal; Litseadeccanensis; Litsealancifolia; Litseaglutinosa; Litseamonopetala; Mice, Methanol Extracts; Back to TOC