Preclinical Evaluation of the Effect of Antioxidant N¬-acetyl-D-Glucosamine on Haematinic Potentials of Lauha Bhasm and Mandura Bhasm
Khobragade D. S1*
, Potbhare M. S2, Lote S. B1, Pardeshi K. S1. Wankhede S. B1 and Tenpe C. R31Datta Meghe College of Pharmacy, Datta Meghe Institute of Medical Sciences(Deemed to be University), Sawangi(M), Wardha, Maharashtra, India.
2GITAM Institute of Pharmacy, Vizag, Andhra Pradesh, India.
3Govt. College of Pharmacy, Amravati, India.
Corresponding Author E-mail: deepak.pharmacy@dmimsu.edu.in
Abstract:
The effect of antioxidant N-acetyl-D-Glucosamine on haematinic activity of two ayurvedic haematinic preparations Lauha bhasm and mandura bhasm was studied in albino rats. Anaemia was induced by administration of phenyl hydrazine (80 mg/kg for 24 hrs) by intra-peritoneal injection. Changes in body weight, red blood cell count (RBC), white blood cell count (WBC), haemoglobin (HB) concentration and haematocrit (HCT) were analysed as indices of anaemia. The mean cell haemoglobin (MCH), mean cell volume (MCV) and mean cell haemoglobin concentration (MCHC) which are indicators of macrocytosis were calculated accordingly. Phenyl hydrazine induced a significant decrease (P<0.05) in the blood parameters indicating anaemia and also resulted to significant increase (P<0.05) in the macrocytosis parameter. Combination of bhasm with antioxidant induced a significant (P<0.05) increase in the RBC count, HBG concentration, and HCT% which had been originally decreased by phenylhydrazine administration. The macrocytosis also turned towards normal as the animals recovered from anaemic condition. The results obtained suggested that combination of antioxidant N-acetyl-D-Glucosamine with Lauha bhasm & mandura bhasm shows improved haematinic activity.
Keywords: Anaemia; Haematinic Activity; Lauha Bhasm; Mandura Bhasm; N-Acetyl-D-Glucosamine; Phenylhydrazine Back to TOC