Biochemical Analysis of the Differential Tissue Expression of Heat Shock Protein70 Following Exposure to Electromagnetic Waves
Nasser Al-Nazwani1*
, Najam Siddiqi2, Yahya Tamimi3 and Zoya Sheikh31Department of Biochemistry, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman.
2Department of Anatomy and Neurobiology, College of Medicine and Health Sciences,National University of Science and Technology, Sohar, Oman.
3Department of Biochemistry, Sultan Qaboos University, Oman.
Corresponding Author E-mail: nassernazwani@nu.edu.om
Abstract: Low field electromagnetic radiation that is emitted from cell phones may induce morphological aberrations and change in gene expression of heat shock protein 70 (Hsp 70). Using Real Time PCR (RTPCR) and protein concentration methods, we studied changes in levels of Hsp70 mRNA and protein synthesis after exposure to the physical stimulus. We also evaluated the suitability of using chick embryo as a model to study alteration in heat shock proteins expression and translation using specific egg incubator set up. Zero-day fertilized chicken eggs were used and a popular mobile phone and service provider was selected with 1800 MHz frequency, power of 0.47 W/kg body and SAR 1.10 w/KG (head). The total daily exposure duration was 50 minutes in each 24 hours with variable maximum exposure time. Experimental samples were divided into controls with no exposure and experimental samples. The developing embryos were removed and different organs were isolated for estimation of Hsp70mRNA and protein. This physical stimulus caused a sequential increase of mRNA corresponding to the period of exposure. Liver tissue demonstrated higher levels of mRNA than heart tissue. Nonetheless, this increase in mRNA was not matched with an increase in the amount of protein of the corresponding mRNA in both tissues at different exposure times. Total protein in all tissues remained unchanged due to slow down of the clearance process and as an indication that the cell is struggling to preserve housekeeping proteins signaling attempt of survival.
Keywords: Cellular Stress; Chick Embryo; Electromagnetic Waves; Extracellular Mrna; Hsp70; Mrna Hsp70; Real Time PCR Back to TOC