YinYang 1 (YY1) and P53 Gene Expression Analysis in Cervical Cancer and Its Relationship with Cancer Staging

Jacobus Jeno Wibisono^1,2, Syahrul Rauf³, Mochammad Hatta^4*, Rosdiana Natsir⁵, Muh Nasrum Massi⁴, M. Husni Cangara⁶, Rina Masadah⁶, Ilham Jaya Pattelongi⁷ and Jonathan Salim²

¹Post Graduate Program of Medical Science, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia.

²Department of Obstetrics and Gynecology, Faculty of Medicine, Pelita Harapan University and Siloam Hospitals Lippo Village, Tangerang, Indonesia

³Department of Obstetrics and Gynecology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

⁴Molecular Biology and Immunology Laboratory, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

⁵Department of Biochemistry, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

⁶Department of Pathological Anatomy, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

⁷Department of Biostatistics, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

Corresponding Author E-mail: hattaram@yahoo.com

Abstract: Background and Aim: Cervical cancer is the global 2^nd most prevalent cancer with ± 266 000 related deaths. Yin Yang 1 (YY1) is a ubiquitous and multifunctional zinc-finger transcription factor in controlling cell cycle. Similarly, p53 suppresses tumors by selectively inhibiting carcinogenesis. The interventional study aims to evaluate YY1 and p53 gene expressions in cervical cancer, serving for further treatment and intervention. Methods: The study measured YY1 and p53 mRNA gene expressions by quantitative real-time PCR through blood sample, which was analyzed by t-test and correlation. Moreover, demographics and medical history were acquired through interviews and medical records. 20 samples are selected by random sampling from July to September 2016. Results: YY1 and cervical cancer stage has negative correlation (r: -0.5) due to its inhibition of cell growth until a certain phase of cancer cell development. While, positive correlation between p53 and cancer stage (r: 0.47) was found because of p53 gene dysregulation. YY1 and p53 correlation are negative (r: -0.9) due to their conflicting functions. Conclusion: The study describes the correlation between YY1 and p53 gene expressions in cervical cancer stages. It was found that the downregulated YY1 expression in cervical cancer as the stage progress is directly opposed to the p53 gene trend. The higher YY1 and lower p53 gene expressions dictate the likelihood of surgery. Therefore, YY1 and p53 can be utilized as a cervical cancer progression indicator and surgery indicator.

Keywords: Cervical Cancer; mRNA Expression; p53 Gene; Staging; YY1

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