Evaluation of Genotoxic and Lipid Peroxidative Potential of Ceftriaxone
Kunjumon Dayana1
and Megaravalli R Manasa2*1Pharmacology Department, Pushpagiri Institute of Medical Sciences and Research Centre, (Kerala University of Health Sciences), Thiruvalla, Kerala, India
2Pharmacology department, Karwar institute of medical sciences, (Rajiv Gandhi University of Health Sciences), Karwar, Karnataka, India
Corresponding Author E-mail : dr.manasamr@gmail.com
Abstract: Lipid peroxidation can produce DNA adducts that can result in genotoxicity. It is involved in pathophysiology of various diseases and drug induced toxicities. Several cephalosporins are reported to cause chromosomal aberrations. Hence this study was planned to evaluate the genotoxic and lipid peroxidative potential of Ceftriaxone in Wistar rats. Ceftriaxone was given at the dose of 500 mg/kg body weight and 1000 mg/kg body weight intraperitoneally to Wistar rats. Genotoxicity was tested by performing in vivo micronucleus test. The frequency of micronucleated polychromatic erythrocytes (%MnPCEs) and polychromatic erythrocytes to normochromatic erythrocytes ratio (PCE:NCE) were estimated. Lipid peroxidative potential was assessed by estimating TBARS (Thiobarbituric acid reactive substance) levels in plasma, erythrocytes and tissue. The activities of antioxidant enzymes were also estimated. The data was analyzed using ANOVA and Dunnett’s test as post hoc. Ceftriaxone at both doses did not increase the % MnPCEs and PCE: NCE ratio in Wistar rats. Ceftriaxone at the dose of 500 mg/kg body weight has significantly altered TBARS levels in erythrocytes. But at a dose of 1000 mg/kg body weight, it has significantly increased plasma, erythrocyte and tissue TBARS levels. The activity of SOD was decreased significantly by ceftriaxone at both doses. The activity of GSH was decreased significantly by ceftriaxone at a dose of 1000 mg/kg body weight. Our study demonstrates that Ceftriaxone does not have the potential to cause genotoxicity. However, it does induce lipid peroxidation and alter the activities of antioxidant enzymes in Wistar rats.
Keywords: Ceftriaxone; Genotoxicity; Lipid Peroxidation; Wistar Rats Back to TOC