Anticancer Activity of Cissus Quadrangularis L. Methanolic Extract Against MG63 Human Osteosarcoma Cells – An In-Vitro Evaluation using Cytotoxicity Assay

Parepalli Suresh¹, Alphienes Stanley Xavier*¹, Karthik V. P¹ and Punnagai K¹

Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai - 600116, Tamil Nadu, India.

Corresponding Author E-mail: alphclinpharm@sriramachandra.edu.in

Abstract: Cissus quadrangularis has wide spectrum of benefits in medical conditions including bone disorders. Alcoholic extract of the plant displayed anticancer activity against cell lines derived from cervical, skin, colon, breast, as well as kidney cancers, and flavonoid fraction of the extract was found to be the active constituent for the activity. To evaluate the anticancer effects of Cissus quadrangularis leaf extract against MG63 human osteosarcoma cell line. MG63 cells were obtained from NCCS, Pune. The methanolic extract of Cissus quadrangularis was prepared and its anticancer activity was tested in cell lines using Mossman method of cytotoxicity assay. The cell viability of MG63 cells ranged between 29.65% and 73.59% at an extract concentration from 1000µg/ml to 7.8µg/ml. The IC₅₀ of extract revealed by this cytotoxicity assay was around 100 µg/ml. This study showed anticancerous activity of C.quadrangularis leaf extract against MG63 cells, which can be further characterized by future studies and aid in treatment of bone tumors.

Keywords: Bone Tumor; Cancer; Cissus Quadrangularis; Methanolic Extract; MTT Assay

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