Antibacterial Effect of Hydro-extract of Pistacia atlantica on Bacteria in In vitro
Iraj Pakzad1,2*, Reza Azizian1, Farid Azizi Jalilian1,2, Morovat Taherikalani1,2, Nourkhoda Sadeghifard1,2 and Mojdeh Mahdi Kartalaie31Deparment of Microbiology , Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
2Research Committee, Ilam University of Medical Sciences.
3Deparment of Epidemiology, Faculty of Medicine, Ilam University of Medical Sciences.
Abstract: Antibacterial resistant microorganisms as agents which cause infection diseases are fourth cause of mortality throughout the world, therefore there is a huge attempt to deal with this issue. New antimicrobial agents design based on high bactericidal potential, high specificity and low cross-reaction. Herbal remedy as an ancient treatment is considerable. Previously, herbal extract were exploited to treat infections such as; Aloa vera, Thymus vulgaris, Allium sativum. This study is aimed to investigate the effect of Pistacia atlantica on bacteria and compare its effect with routine antibiotics. Sample size was determined 30 numbers per each bacteria by STATCALC software (EPI-Info). Hydro-extract prepared from Pistacia atlantica that collected from Zagros mountains. Disck diffusion and well embedding method recruited to investigate antibacterial effect. MIC test done for hydro-extract and antibiotics. Statistic analysis done by Square Chi test, t independent test, t-test and fisher test. MIC of Pistacia atlantica extract were 163µg/ml, 104.16µg/ml and 204.67µg/ml for E.coli, Pseudomonas aeruginosa and Staphylococcus aureus, respectively. These funding shown inhibition effect of this herbal extract but it indicate no affect on H.pylori. Funding indicate inhibit effect of hydro-extract on recruited bacteria except H.pylori thereby, it is suggested that phenol/chloroform-extracion could be have inhibit affect on H.pylori.
Keywords: H.pylori; Staphylococcus aureus; Pseudomonas aeruginosa; E.coli; Pistacia atlantica; Hydro-extraction Back to TOC