Heteroxenia Ghardaqensis Extract Protects Against DNA Damage in Streptozotocin-Induced Experimental Diabetes
Abdel Razik Farrag1, Mahmoud Nassar2, Zakaria El-Khayat3, Jihan Hussein*3, Nadia Ahmed Mohammed3, Dalia Medhat3, Abd El-Nasser El-Gendy4 and Abdelsamed Elshamy2

1Departments of Pathology, National Research Centre, Cairo, Egypt.

2Natural Compounds Chemistry, National Research Centre, Cairo, Egypt.

3Medical Biochemistry, National Research Centre, Cairo, Egypt.

4Medicinal and Aromatic Plants Research Department, National Research Centre, Cairo, Egypt.

Corresponding Author E-mail: jihan_husein@yahoo.com

Abstract: DNA damage is correlated to type-2 diabetes mellitus (T2DM) and its complications via oxidative stress. This study aimed to evaluate the anti-diabetic effect of Heteroxenia ghardaqensis extract on streptozotocin (STZ) induced-diabetes and how far can this extract attenuate DNA damage in this model. Forty male albino rats (160-180 g) were used in this study and divided into four groups: control, diabetic, diabetic rats received H. ghardaqensis extract (30 mg/kg body weight/day) orally for four weeks and diabetic rats received H. ghardaqensis extract (60 mg/kg body weight/day) orally for four weeks. After the experimental period, fasting blood sugar and serum cholesterol were determined. Urinary 8-hydroxyguanosine (8-OHdG) as a marker of DNA damage was estimated by reversed phase (HPLC). Liver and kidney nitic oxide (NO) and malondialdehyde (MDA) were estimated. Pancreatic tissues were histopathologicaly examined. Our results suggested that diabetes mellitus is accompanied by elevation of DNA damage that enhances the tendency to mutagens and reduce the efficacy of DNA repair. H. ghardaqensis extract appeared to be effective against the oxidative stress induced by STZ which may be due to sesquiterpenoids and diterpenes compounds that scavenge free radicals and increase the antioxidant enzymes as appeared in attenuation of DNA damage.

Keywords: Diabetes Mellitus; DNA Damage; Heteroxenia Ghardaqensis; HPLC - 8-Ohdg; Streptozotocin

[ HTML Full Text]

Back to TOC