Genetic Diversity and Dendrogram of Cardiospermum Halicacabum- An In Vitro Study
S. Thiruvengadam
, K. S. Shreenidhi and B. S. Mohan Kumar
Department of Biotechnology, Rajalakshmi Engineering College, Chennai, TN, India.
Corresponding Author E-mail: thiruvengadam.s@rajalakshmi.edu.in
Abstract: Cardiospermum halicacabum commonly known as Balloon plant or Love in a puff, a climbing plant often found in tropical and subtropical Africa and Asia. It is widely used in homeopathic medicine for the treatment of arthritis, purulent discharge and others. This particular plant species is becoming endangered due to their extensive use for commercial purposes and genetic diversity at inter-species level arises due to geographical distribution, climate changes and environmental stress. As an initiative for conservation of this species, the present contemplation was done by collecting DNA samples from leaves of the above mentioned plant which were grown in five different locations in and around Chennai and the investigation of their diversity was done using genetically reliable DNA markers like OPA 5, OPA 9, OPA 10, OPB 18, OPC 7, OPN 7 and OPN 12. RAPD PCR was performed. It provided providing information at molecular level and the total number of bands formed, designate the presence of genetic variations among species in different location. Dendrogram was constructed using scoring methods found the differences among the same species arising from evolution, it could be environmental change and technological developmental impacts.
Keywords: Arthritis; C.halicacabum; Dendrogram; Genetic Diversity; PCR Back to TOC