Effect of Co Enzyme Q10 Alone or in Combination With Vitamin C on Lipopolysaccharide-Induced Brain Injury in Rats

Nabila A. El-Laithy¹, Elsayed M. E. Mahdy², Eman R. Youness¹, Nermeen Shafee³, Mohamed S.S. Mowafy⁴ and Mahmoud M. Mabrouk¹  

¹Department of Medical Biochemistry, National Research Centre, Cairo, Egypt.

²Department of Chemistry , Faculty of Science, Helwan University, Helwan,  Egypt.

³Department of Pathology, National Research Centre, Cairo, Egypt.

⁴Critical care unit, Faculty of Medicine, Cairo University, Cairo, Egypt.

Corresponding Author E-mail: hoctober2000@yahoo.com

Abstract: Our was to determine the impact of CoenzymeQ10 (Co Q10) and vitamin C alone or in combination on oxidative stress in brain tissue of rats during endotoxemia induced by single intraperitoneal dose of Lipopolysaccharide (LPS), 500µg/kg. Both CoQ10&vitamin C were given orally to rats with doses (200&100 mg/kg) respectively for 7successive days prior induction of endotoxemia .LPS injected, with Co Q10 with doses (100 &200 mg/kg) &vit. C (50&100 mg/kg).In addition CoQ10 and vitamin C together in doses (100&50 mg/kg) & (200&100 mg/kg) respectively were added to LPS-treated rats. Then euthanized 4 hours later. Histopathological assessment of brain tissue was done. Results: LPS injection induced oxidative stress in brain tissue, resulting in marked increase in malondiadehyde (MDA), nitrite (NO) and Amyloid beta (Aβ), while decreasing reduced glutathione (GSH), paraoxonase-1 (PON1) and brain derived neurotrophic factor (BDNF).CoQ10 and vit.C administration with doses(200&100 mg/ kg) before endotoxemia result in reduction of brain MDA, NO and Aβ, while increasing levels of GSH, PON1 and BDNF compared to controls. The addition of both Co Q10 &vit.C to LPS- treated rats lead to decrease of brain  NO, MDA and Aβ, also  increase of GSH, PON1 and BDNF. This effect was more obviouswith high doses, this due to the ameliorating effect of both CoQ10 and vit.C on oxidative stress of brain tissue during endotoxemia.This consisted with the histopathological results. Conclusion: this work focuses on the possible role of CoQ10 &vit.C as antioxidants in protecting brain tissue.

Keywords: Co Q10-vitamin C-LPS-oxidative stress-Aβ-BDNF

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