Study on PIK3CA Gene Mutations in Oral Squamous Cell Carcinoma Among South Indian Populations
Jayalalitha Sathiyamoorthy1, Vidyarani Shyam Sundar2, N. Aravindha Babu2, Subbaih Shanmugham3, Jagadeesan.G.Mani3, Ponnuraja Chinnaiyan4, Aparna Kalyanaraman1 and Rajeswary Hari1
1Department of Biotechnology, Dr. M.G.R Educational and Research Institute Maduravoyal, Chennai-95, India.
2Centre of Oral Cancer Prevention and Research, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education (Bharath University), Pallikaranai, Chennai-99, India.
3Centre of Surgical Oncology Government Royapettah hospital and Kilpauk Medical College, Chennai, India.
4Department of Statistics, National Institute of Research in Tuberculosis, No:1, Sathiyamoorthy Road, Chetpet, 600031,Chennai, India.
Corresponding Author E-mail: jayalalitha.vit@gmail.com
Abstract: The present investigation was performed in South Indian Populations to determine the hotspot mutation frequency in Oral Squamous Cell Carcinoma (OSCC) patients with PIK3CA gene Exon 9 and Exon 20 and its correlations with help of their clinical characteristics leading to these mutations. PI3KCA belongs to a group of regulatory heterodimeric lipid kinase which is involved in proliferation of cells, apoptosis and as well in metastasis which is controlled by PIK3CA gene is subjected to high frequency of somatic mutation in various tumors including OSCC. Total of 25 OSCC patients samples comprising of male and female subjects from Government tertiary care Centre were included in this study. Tumor specimen samples were collected and amplified for PIK3CA gene by PCR and subjected to genomic DNA Sequencing. Our findings showed total of 20% of oncogenic frequency in PIK3CA gene. We also observed two hot spot mutations (E545K) in exon 9 gene and three hot spot mutations (H1047Q, H1047Y, H1048Q) in exon 20 gene in our study populations. 0 Based on our findings it may be concluded that PIK3CA gene Exon 9 and Exon 20 contributes to a major role in pathogenesis on OSCC among South Indian populations may act as therapeutic target for a anticancer drug for the treatment OSCC.
Keywords: hotspot OSCC, PIK3CA, tumor, Back to TOC