Effects of Exercise Training on Development of Breast Cancer in Mice
Anoosheh Leila1*, Kordi Mohammad Reza2, Gaeini Abbasali2, Mahdian Reza3, Mirakhori Zahra11Exercise Physiology Ph.D., Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran 2Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran 3Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Corresponding Author Email : l.anoosheh@ut.ac.ir
Abstract: The purpose of the present research was to determine the effects of aerobic exercise on NF-kB, Lin28B, let-7a microRNA and IL-6 of tumor tissue in mice with breast cancer. 20 female BALB/c mice were injected with MC4-L2 estrogen-receptor-positive breast-cancer cells, then divided into two groups of 10 mice each: tumor-exercise (TE) and tumor-control (TC). The TE group performed aerobic exercise five days per week for six weeks. Widths and lengths of the tumors were measured weekly using digital calipers. The mice were sacrificed 48 hours after the last exercise session. Expression of NF-kB, Lin28B and miRNA let-7a genes were quantified with Real time-PCR and IL-6 was measured by the ELISA test. Tumor volume, expression of NF-kB and Lin28B genes and IL-6 levels diminished significantly in the TE group compared with the TC group, but let-7a microRNA expression showed a significant increase in the TE group compared with the TC group (P<0.05). The results demonstrate that aerobic exercise can have an effective role in the diminution of IL-6, Lin28B and NF-kB, and the increase of microRNA let-7a and it seems that regular aerobic training may at least have a therapeutic role in estrogen-receptor-dependent breast cancers. However, more studies are needed.
Keywords: Estrogen-receptor-dependent breast cancer; Aerobic training; NF-kB; Lin28B; microRNA let-7a Back to TOC