Gene Expression and Clinical Manifestations in Iraqi Patients with Acromegaly
Taghreed Uloom Mohammed
Chemistry Department- College of Education for Pure Science (Ibn Al-haitham), Baghdad University.
Corresponding Author E-mail: tagreedaloom@gmail.com
Abstract: Acromegaly is a disease characterized by growth hormone and insulin like growth factor hypersecretion due mostly to pituitary somatotropic adenoma. The diagnosis of Acromegaly is typically deferred for years exposing patients to slowly progressing chronic complications. The present study consist of two parts, the part one was aim to study the lipid, lipoprotein profiles, insulin resistance and enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase) of 30 patients with actively secreting growth hormone (15 female and 15 male; aged 30-50 years). The other part is intended to analysis the GH1 gene mutation in Iraqi patients with growth hormone-producing pituitary tumors causing acromegaly. Genomic DNA was insulated from the patient and 10 healthy controls, and arranged for polymerase chain reaction analysis. Several pairs of primers were intended to amplify exons 1-5 of the growth hormone 1 gene and the polymerase chain reaction produced were sequenced to detect mutation.
Keywords: Gentic Study; Acromegaly; Lipid Profile; Insulin like Growth Factor-1 Back to TOC