Topology Prediction and Motifs Identification of Bicyclomycin Resistance Protein of Ehrlichia Canis Strain Bangkok
Arinthip Thamchaipenet¹*, Surin Peyachoknagul¹, Sathaporn Jittapalapong² and Danai Pinyoowong³¹Department of Genetics, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand,
²Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
³Department of Small Animal and Radiology, Faculty of Veterinary Medicine, Mahanokorn University of Technology, Nongchok, Bangkok 10530, Thailand.
Abstract: Bicyclomycin resistance gene from E. canis strain Bangkok was isolated by polymerase chain reaction using primers designed from the genome sequence of E. canis strain Jake. The hypothetical Bcr protein was analysed and revealed close relationship with those of drug resistance transporter Bcr/CflA subfamily from bacteria in order Rickettsiales. Topology prediction using hidden Markov model algorithms indicated that the E. canis strain Bangkok Bcr protein was an 11-transmembrane segment protein with N-terminal out of cell and C-terminal in the cytoplasm. It contained special motifs conserved in drug transporters belonging to major facilitator superfamily (MFS). Phylogenetic analysis showed that hypothetical Bcr proteins of rickettsia species were segregated from the 12-TMS proteins and were likely to represent a new member of MFS.
Keywords: E. canis strain Bangkok; bicyclomycin; drug resistance transporter Bcr/CflA subfamily Back to TOC