Effect of Quinoline-2-One Derivatives on the Gene Expression of Mexb of Pseudomonas Aeruginosa
Essam Fadel Al-Jumaily and Nagham Qasim AbdBiotechnology Department Genetic Engineering and Biotechnology Institute for postgraduate studies, University of Baghdad, Iraq.
Corresponding Author E-mail: Samgen992003@Yahoo.com
Abstract: Sixty isolates of Pseudomonas aeruginosa were collected from different hospitals of Baghdad. Detection of mexB gene in the isolates was done by using PCR technique and the gene was found in all of the isolates.The susceptibility of the isolates was tested against Carbenicillin, Levofloxacin and Erythromycin by using disc diffusion method.Results showed that 80% of the total isolates was resistant to Carbenicillin while 6% and 32% of the total isolates was resistant to Levofloxacin and Carbenicillin respectively.Three isolates that were resistant to the three antibiotics were chosen to be the base for detecting the gene expression level of mexB before and after the addition of Quinoline-2-one Derivatives.
Keywords: Carbenicillin; Erythromycin; Levofloxacin; mex Bgene; Pseudomonas aeruginosa; Quinoline-2-one Derivatives Back to TOC