Optimization of Media Components and Growth Conditions to Enhance Lipase Production by Pseudomonas sp. Lp1
S. Kanimozhi1* and K. Perinbam21Sathyabama University, Jeppiaar Nagar, Old Mamallapuram Road, Chennai - 600 119 India.
2Faculty of Biomedical Engineering, Sathyabama University, Jeppiaar Nagar, Old Mamallapuram Road, Chennai - 600 119 India.
Abstract: Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. Lipases occur widely in nature, but only microbial lipases are commercially significant. In the present study, production of extracellular lipase in submerged fermentation of Pseudomonas sp.Lp1 isolated from oil contaminated soil has been investigated. The lipase production was optimized in shake flask experiments. The objective of the present investigation is to determine the activity of lipase was under varied physiochemical conditions of incubation time, pH, temperature, lipid carbon sources, nitrogen sources, surfactants and cations. The use of natural substrates in lipase production by Pseudomonas sp.Lp1 was also studied. The extracellular lipase production was maximum during the incubation time of 36 to 48 hours. The observed pH and temperature range optimum for maximum lipase production were 8.0-8.5 and 37-42 °C, respectively. Maximum lipase production occurred when olive oil (1%) was used when compared with Coconut oil, Sun flower oil Corn oil and Ground nut oil. Among the surfactants tested such as Triton X-100, Tween-20, Tween-80, Sodium dodecyl sulphate, all of them showed positive influence on lipase production,. Lipase activity was recorded to be the maximum when yeast extract and peptone were used as nitrogen source. About 45U/ml of enzyme activity was obtained in the medium prepared with whey as the natural substrate (2%).
Keywords: Lipase; Pseudomonas; Natural substrates; Production; Medium optimization Back to TOC