Separation of Cancer Proteins using Polyacrylamide Gel Electrophoresis
J. Malathi* and P. Somashekhar
Department of Microbiology, Shivani college of Pharmacy, Warangal - 506 001, India.
Abstract:
The cancer is major disease which is convulsing all the global, so many of hazardous effects carried by this disease. Normally the cancer development occurs due to unregulated body metabolisms, Mutations, Uncontrolled cell growth, some of the proteins also involved in development of cancer disease. The oncogenes are plays vital role in expansion of cancer, normally most cancers are somatic, not germ line mutational events, although some cancers have heritable form, cancer occurrence is sporadic in most cases. Over time, several somatic mutations accumulate with in the same somatic cell lineage, altering or knocking out the functions of several genes, until finally a cancerous cell expand in to a clone of cancerous cells. Addition to this so many of mechanisms converting normal cell in to cancer cell sequential biochemical events depend on the successful occurrence of prior events, in the cell cycle, fail-safe mechanisms prevent the cell cycle from progressing until prior events have been successfully completed, survival factors block advancement of the death pathways. The magnification of cancer is initiated by the various components of the intercellular signaling systems, modified by protein phosphorylation, allosteric interactions between proteins and small molecules, and interaction between protein subunits. The variation also occurs in the amount of albumins and globulins from a normal cell to cancerous cell, where as all the proteins were involved in improvement of disease. The present work is conducted to know the different amounts of serum albumins and globulins present in the cancer patients, for this we compiled 15 positive cancer samples from Warangal district hospital, and subjected to Organic solution precipitation and salt precipitation. Subsequently these precipitated protein samples were analyzed by SDS Polyacrylamide gel electrophoresis, and different bands of albumin and globulin seen with different concentrations in a densitometer.
Keywords:Plasma proteins; a; b; g Globulins; Precipitation; Coomassie blue staining; SDS-PAGE
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