linkedin
Mor P, Rathore A. R, Sonagra V, Sharma N, Sharma A. A Study of Serum Copper, Zinc and Magnesium in Type 2 Diabetes Mellitus with Complications and without Complications.Biomed Pharmacol J 2020;13(4).
Manuscript received on :15-Jul-2020
Manuscript accepted on :3-Dec-2020
Published online on: --
Plagiarism Check: Yes
Reviewed by: John George Hardy
Second Review by: Tukaram Dudhamal
Final Approval by: Dr. Francesca Gorini
How to Cite    |   Publication History
Views Views: (Visited 1,119 times, 1 visits today)   Downloads PDF Downloads: 413

Priyanka  Mor1, Anjana Kanwar Rathore1 , Vanita Sonagra1, Neha Sharma2*and Ashish Sharma2

1Department of  Biochemistry,  Dr.S.N. medical college, Jodhpur, Rajasthan. India.

2Department of  Biochemistry,  Geetanjali medical college and hospital, Udaipur, Rajasthan, India.

Corresponding Author E-mail: neha16.sharma@gmail.com

DOI : https://dx.doi.org/10.13005/bpj/2070

Abstract

In 21st century Type 2 diabetes mellitus is most challenging problem for human being. Trace elements are known as important substances for human health, because of metabolic characteristics and their functions. They have role in catalytic and regulatory functions. Trace elements interact with  enzymes, pro hormones and pre secretory granules ,  biological membranes. Among the trace elements Copper (Co), Zinc (Zn) and magnesium (Mg) are most valuable. The present study has been planned to verify the role of these particular trace elements in diabetic complications and to find out the relation between these if there is any. copper, zinc and magnesium in relation to various complications of type 2 diabetes mellitus. This study was done in geetanjali medical college and hospital on 120 patients with healthy control. Present study has shown increased Cu levels, and decreased level of Zinc and magnesium in comparison to control group. An adequate supply of particular substances in the diet of these diabetic patients can be effective in the management of diabetic patients.

Keywords

Cardiovascular Disease; Diabetic Nephropathy; Fasting Plasma Glucose; Trace Elements

Download this article as: 
Copy the following to cite this article:

Mor P, Rathore A. R, Sonagra V, Sharma N, Sharma A. A Study of Serum Copper, Zinc and Magnesium in Type 2 Diabetes Mellitus with Complications and without Complications.Biomed Pharmacol J 2020;13(4).
Copy the following to cite this URL:

Mor P, Rathore A. R, Sonagra V, Sharma N, Sharma A. A Study of Serum Copper, Zinc and Magnesium in Type 2 Diabetes Mellitus with Complications and without Complications.Biomed Pharmacol J 2020;13(4). Available from: https://bit.ly/3a1jSyk

Introduction

A big health problem of present era is Type 2 diabetes mellitus (1). The presence of  metabolic imbalance  lead to unchanged  irreversible functional and structural alteration  in body which  lead towards “diabetic complications”, which affecting,  cardiovascular, eye, renal and nervous system mostly (2) Chronic effect of  type 2 diabetes could divided into vascular and nonvascular complications.  Diabetic vascular complications  are  subdivided into micro and macro vascular complications.  (3) .  Few of them act as antioxidants. They prevent peroxidation of membrane  Similarly on glucose metabolism.(4)  Study regarding  these  elements has been continuously increasing during last two decades .    they are known as  essential for  health of human being because of their various  functions in metabolism and cell structure.  They have an important role in functions of cell membrane, as enzymes and hormones..(5) In between  Copper (Co), Zinc (Zn) and magnesium (Mg) are of.  Important (6) copper has insulin–like activity and increasing lipogenesis. (7)

So many studies showed changes in serum copper level associated with diabetic patients.(8) Zn will be able to affect capability of islet cells  of pancreas . Which are responsible for insulin production and secretion in type-2 diabetes (9). Metabolism of glucose and transporting mechanisms are affected by magnesium as a cofactor   by affecting enzyme involve in insulin secretion and their binding capacity. (10) We have planned this study to identify the role of trace elements in diabetic complications and to find out their relation.

Material and Methods

Cases were clinically diagnosed cases of type 2 DM with and without complications attending the biochemistry, ophthalmology, nephrology and cardiology department at Geetanjali Medical College & Hospital, Udaipur (Rajasthan).

The total sample of 120 patients including cases and control was taken in which equal division had done as 30 control and 30 in each complication.

Group 1- Clinically diagnosed cases of DM type 2 without complications

Group 2- Clinically diagnosed cases of DM type 2 with retinopathy

Group 3- Clinically diagnosed cases of DM type 2 with nephropathy

Group 4- Clinically diagnosed cases of DM type 2 with coronary artery diseases

Informed consent was obtained from all subjects for participating in the study. Blood sample was collected by vein puncture using an aseptic technique. Serum was Separated from the sample and was analyzed for following biochemical parameters- copper wes estimated by Di-Br- PAESA method, serum zinc and magnesium by Calmagite method and 5-Brom-PAPS Johnsen and R.Eliasson method, respectively done Erba chem. V2 5 plus semi automated analyzer.

Results

Present study has shown increased Cu levels, and decreased level of Zinc and magnesium in comparison to control group.

 Table 1: comparisons between the patients with diabetic complications and Control group for Copper

S.NO. PARAMETER TYPES OF COMPLICATION          MEAN±- SD(N=30) P VALUE
COPPER

(μg/dL)

 PATIENTS CONTROL
1 DIABETIC NEUROPATHY 125 ± 120.52 103 ± 7.08 <0.0001*
2 CARDIOVASCULAR DISEASE 12 9± 12.52 103 ± 7.08 <0.0001*
3 DIABETIC RETINOPATHY 129 ± 14.3 103 ± 7.08 <0.0001*

Table 2: comparisons between the patients with diabetic complications and Control group for Zinc.

S.NO. PARAMETER TYPES OF COMPLICATION          MEAN±- SD(N=30) P VALUE
ZiNC

(μg/dL)

 PATIENTS CONTROL
1 DIABETIC NEUROPATHY  56 ± 6.7 67.6 ± 6.23 <0.0001*
2 CARDIOVASCULAR DISEASE 56.8 ± 8.1  67.6 ± 6.23 <0.0001*
3 DIABETIC RETINOPATHY 54 ± 6.09  67.6 ± 6.23 <0.0001*

Table 3: comparisons between the patients with diabetic complications and control group for magnesium.

S.NO.

 PARAMETER TYPES OF COMPLICATION          MEAN± SD(N=30)

P VALUE
MAGNESUIM

(μg/dL)

 PATIENTS CONTROL
1 DIABETIC NEUROPATHY   1.6 ± 0.25 1.92 ± 0.37 0.0002*
2 CARDIOVASCULAR DISEASE 1.7 ±  0.37  1.92 ± 0.37 0.0130*
3 DIABETIC RETINOPATHY 1.73 ± 0.30

 1.92 ± 0.37

0.033*

Discussion

Complications associated with diabetes mellitus  are increasing during last two decades and increasing the rate of mortality in developing and developed countries around the globe..(11)  The role of trace elements are still not clear in diabetic patients, specially copper, Zinc and Magnesium..(12,13) We have determined the level of Copper, zinc and magnesium in the serum of all the patients as well as in controls and finally the results of cases were compared with that of controls. This study has shown increased Cu levels in diabetic patients with complication than without complication. The higher  serum level of  Cu in diabetic patients found in our research was Similar with the study of Ghada A El-Zebda (2006), in their study Copper mean concentration was higher in diabetic hypertensive than control group and not significant, (P>0.05). (14) In contrast to our result M Prasad Naidu, Shiva Kumar et al in 2013 concluded that the mean blood copper levels in Diabetic Nephropathy cases were not significantly differed(15).

We have found significantly low level of Zinc in  patients in comparisons to control. Our study is similar with the study of  Mohan Lal, Sudha K et al (KMC, Manipal) a significant decrease in, Zn and Mg. (16) which is similar to observations reported by Ghada A El-Zebda (2006), mean concentration of zinc was higher than control group and Significant (P<0.05)(14).  Hyperglysuria will lead to low zinc level.  Zn also has an important role as antioxidant and defense oxidative stress. Zinc deficiency lead to damage in cell  and tissues because of oxidative stress in diabetic patients. (Chausmer 1998).(17)

In this study, magnesium levels in diabetics with complications were lower than the control group.  Decrease level of serum magnesium commit to progression of diabetic Complications like retinopathy, abnormal functions of platelet, Cardiac functions , hypertension by decreasing  the inositol transport  pathway and later intracellular depletion(18). Low  level of magnesium in  circulation  lead to  hypertension, abnormal lipid metabolism, increased inflammation , thickness of  carotid wall, oxidative stress  and  heart disease(19,20).

The kidney plays a major role in magnesium homeostasis and in maintenance of magnesium concentration. In addition to osmotic action of glucosuria, hypomagnesemia may also occur following insulin therapy for diabetic ketoacidosis and may be related to the anabolic effects of insulin driving magnesium back into cells. (21, 22)

This study suggests that zinc concentration may be considered a biochemical marker of oxidative stress associated with complications of diabetes. Deficiency of nutrients in developing countries can be a significant contributing factor in progression of chronic diseases, such as diabetes. The data of our study does not allow us to conclude whether zinc and magnesium deficiency and elevated serum copper level are the major causes of complication to occur.

However, the elevated copper level and low zinc and magnesium levels in patients having diabetic complications as compared to the controls indicated there strong association in the Indian population.

Conclusion

This is recommended that an ample of these elements in the diet of diabetic patients can be helpful in the management of diabetic patients in future. As well as antidiabetic therapy, s zinc  , magnesium supplementation  and copper  chelation  can lessen  Complications  because of diabetes mellitus yet still more studies in this concern  are Suggested.

References

  1. Kingh H, Aubert RE, Herman WH. Global burden of diabetes, 1995-2025:prevalence, numerical estimates and projections. Diabetes Care,1998 ;21:1414-1431.
    CrossRef
  2. Koda-Kimble MA, Carlisle BA. Diabetes mellitus. In :Young LY, Koda- Kimble MA, Kradjan WA, Guglielmo BJ, editors. Applied therapeutics: the clinical use of drugs. 6th ed. Vancouver (WA): Applied therapeutics, 1995;48:481-5.
  3. Power AC. Diabetes mellitus. In Braunald E, Fauci AS, Kasper DL, HauserSL, Longo DL, Jameson JL, editors. Harrison’s Principles of Internal nMedicine. 17th ed. New York. McGraw Hill ;2286.
  4. Zargar AH, Shah NA. Copper, Zinc and Magnesium levels in non-insulin dependent diabetes mellitus. Postgrad Med J 1998; 74: 665-668.
    CrossRef
  5. Nourmahammadi I, Reayzi GH, Ghaemghai J. Serum and urine level of Cu,Zn, Mg and Ca in Iranian patients exposed to chemical war gases. J Trace Elem Exp Med 1989; 2:88.
  6. Dormonday TL. Trace elements analysis of hair. BMJ 1986; 293: 975-6.
    CrossRef
  7. Quilliot D, Dousset B, Guerci B, et al. Evidence that diabetes mellitus favours impaired metabolism of zinc, copper, and selenium in chronic pancreatitis. Pancreas 2001; 22: 299–306.
    CrossRef
  8. Kinlaw WB, Levine AS, Morley JE, Silvis SE. Abnormal zinc metabolism intype II diabetes mellitus. Am J Med 1983; 75: 273–7.
    CrossRef
  9. DiSilvestro RA (2000) Zinc in relation to diabetes and oxidative disease. J Nutr 130:1509–1511.
    CrossRef
  10. Gurfinkel D. Role of magnesium in metabolism. Magnesium 1988; 7:249-61.
  11. Karnik ND. Macrovascular complications in diabetes mellitus. The journal of general medicine 2002; 14:15-20.
  12. Jacob RA. Trace Elements. In: Teitz HW, ed. Textbook of Clinical chemistry. Philadelphia: Saunders, 1986; pp 865-94.
  13. Dormonday TL. Trace element analysis of hair. BMJ 1986; 293:975-6
    CrossRef
  14. Ghada A El-Zebda (2006). Significance of serum levels of copper and zinc inType II diabetic, hypertensive, and diabetic hypertensive patients in GazaCity. The Islamic university-Gaza
  15. .M Prasad Naidu1, Shiva Kumar2, S Mahaboob Vali1, Desai Madhav3 and GSubrahmanyam1 2013.
  16. Lal Mohan, Sudha K and Shetty, Beena V and Rao, Gayathri M. Influence of modified levels of plasma Magnesium, Cu, Zn and Iron levels on Thiols and protein status in diabetes mellitus and diabetic retinopathy. International journal of Analytical, Pharmaceutical and Biomedical sciences,2013;2 : 67-72.
  17. Chausmer AB (1998) Zinc, insulin and diabetes. J Am Coll Nutr 17:109–115.
    CrossRef
  18. Alzaid A, Moyer T, Rizza R. Effects of insulin on plasma magnesium in non insulin dependent diabetes mellitus. J Clin Endocrin Metab 1995;80:1376-81.
    CrossRef
  19. Rodriguez-Moran M, Guerrero-Romero F. Low serum magnesium levels and foot ulcers in subjects with type 2 diabetes. Arch Med Res 2001;32:300–303.
    CrossRef
  20. Mandal G. K.. Comparative study of microalbuminuria and glycated Hemoglobin levels in Type-2 diabetic complications. Asian J Pharma Clin Res, Vol 9 (2) 2016: 356-360.
  21. Ohira T, Peacock JM, Iso H, Chambless LE, Rosamond WD, Folsom AR.Serum and dietary magnesium and risk of ischemic stroke: the Atherosclerosis Risk in Communities Study. Am J Epidemiol 2009;169:1437–1444.
    CrossRef
  22. Fujii S, Takemura T, Wada M, Akai T, Okuda K. Magnesium levels in plasma erythrocytes and urine in patients with diabetes mellitus. Horm Metab Res. 1982;14:61–2.
    CrossRef
Share Button
(Visited 1,119 times, 1 visits today)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.