Enzymatic Method for the Determination of Perindopril Erbumine and Repaglinide in Bulk and Dosage Forms
A. Prameela Rani ¹*, C. Bala Sekaran² and Ch. Srilakshmi³¹Department of Pharmaceutics, K.V.S.R. Siddhartha College of Pharmaceutical Sciences, Vijayawada - 520 010 India. ²Department of Biotechnology, Jagarlamudi Kuppuswamy Chowdary College, Guntur - 522 006 India. ³Department of Biochemistry, P.B.Siddhartha College of Arts and Science, Vijayawada - 520 010 India. Corresponding Author E-balumphil@gmail.com
Abstract: Two simple and sensitive enzymatic methods were developed for the determination of Perindopril erbumine (method A) and Repaglinide (method B) either in raw material or in pharmaceutical formulations. Both methods A and B are based on oxidative coupling reaction between drug and 3-methylbenzothiazolin-2-one hydrazone (MBTH) in the presence of Hydrogen peroxide and enzyme horseradish peroxidase to produce colored product, which is measured spectrophotometrically at 425nm and 440nm respectively. Beer’s law is obeyed in the concentration range of 10-50 µg/ml and 8-40 µg/ml for method A and method B respectively. The proposed methods were successfully applied to the assay of perindopril erbumine and repaglinide in tablet preparations with recoveries varying from 99.50 to 100.50% and 99.75 to 100.15%, with standard deviation of 0.0015 for method A and 0.00118 for method B. The results were statistically compared with those of the reference method. No significant interference was observed from the excipients commonly used as pharmaceutical aids with the assay procedure.
Keywords: Perindopril erbumine; Repaglinide; Sandell’s sensitivity; Beer’s Law Back to TOC