Spectrophotometric and HPLC Methods for Simultaneous Estimation of Cefadroxil and Ambroxol from Tablets.
Esther Roset Shobana¹ and Lakshmi Sivasubramanian²

¹Department of Pharmaceutical Chemistry, Vellore Institute of Technology, Vellore  (India)

²Department of Pharmaceutical analysis, SRM college of Pharmacy, SRM University, Kattankulathur (India).

Correspondence Author E-mail: lakshmiss@hotmail.com

Abstract: A simple, accurate, economical and reproducible UV spectrophotometric and a HPLC method for simultaneous estimation of two-component drug mixture of cefadroxil and ambroxol in combined tablet dosage form have been developed. The first developed method employs multiwavelength spectroscopy using six mixed standards and 230.0 nm and 245.0 nm as two wavelengths for estimation. Linearity was observed in concentration range of 10-60 µg/ml of cefadroxil and 1.2-7.2 µg/ml of ambroxol. Developed HPLC method is reverse-phase chromatographic method using Hypersil C18 column and acetonitrile:phosphate buffer (pH 5.0) in the ratio of 10:90 v/v as mobile phase. For HPLC method, linearity was observed in concentration range of 100 –500 µg/ml of cefadroxil and 12.5-62.5 µg/ml of ambroxol. Results of analysis were validated statistically and by recovery studies.

Keywords: HPLC; Cefadroxil; Ambroxol tablets

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