Cytotoxic Evaluation of Cisplatin, Cisplatin/Hydroxypropyl- b-Cyclodextrin Complexes and their Nanoparticles Drug Delivery Systems.
Anna Balaji1*, V. P. Pandey2, R. Manavalan2 and M. S. Srinath31Department of Pharmaceutics, Dayananda Sagar College of Pharmacy, Bangalore (India).
2Department of Pharmacy, Annamalai University, Annamalainagar (India).
3Department of Pharmacy, CMR College of Pharmacy, Bangalore (India).
Abstract: Our study reported herein utilized the Agrobacterium tumefaciens–induced potato disc tumor assay. The objective was to detect of extent of antineoplastic activity in the potato disc tumor induction assay by Cisplatin, its HP-b-CD complexes and gelatin nanoparticles loaded with Cisplatin and its HP-b-CD complexes. Tumor inhibition was seen in all the tested drugs and its formulations. The order of activity was: 1:2 Cisplatin/HP-b-CD > 1:1 Cisplatin/HP-b-CD > Cisplatin > 1:2 Cisplatin/HP-b-CD loaded nanoparticles >1:1 Cisplatin/HP-b-CD loaded nanoparticles > Cisplatin loaded nanoparticles. The A. tumefaciens–induced potato disc tumor assay was an effective indicator of the extent of antitumor activity regardless of the mechanism of drug action. In the brine shrimp lethality bioassay (BSLT), the Medium Lethal Concentrations (LC50 value) of Cisplatin and Cisplatin/HP-b-CD inclusion complexes were determined using Artemia salina L. (Artemiidae) with the objective was to compare the LC50 values of the pure drug, its HP-b-CD complexes and loaded gelatin nanoparticles to determine their relative degree of cytotoxicity. The bioassay results showed significant dose dependent mortality. Cisplatin, 1:1 Cisplatin/HP-b-CD and 1:2 Cisplatin/HP-b-CD inclusion complexes, Cisplatin loaded nanoparticles, 1:1 Cisplatin/HP-b-CD loaded nanoparticles and 1:2 Cisplatin/HP-b-CD loaded nanoparticles exhibited potent brine shrimp lethality with LC50 of 106.71, 83.43, 76.53, 270.96, 177.59 and 149.55 µg/mL respectively. Thus, potato disc assay would be acceptable as a primary general screen for antineoplastic activity of various complexes of Cisplatin and its formulations regardless of mode of inhibitory action on tumor formation. The present study also supports that brine shrimp bioassay is simple, rapid, reliable and convenient method for assessment of degree of cytotoxicity.
Keywords: Cisplatin; Cisplatin/Hydroxypropyl-b-Cyclodextrin inclusion complex; antitumor; cytotoxic Back to TOC