Investigating the Effect of Borago Officnale on Hipocampal IL-1 Beta Protein and Gene in the Amyloid Β-Peptide (25–35)-Induced of Inflammation in Rat
Ehsan Barati*1, Sara Soleimani Asl2, Seyed Ali Pourbakhsh3, Mahmoud Jamshidian3 and Siamak Shahidi4

1Student of Veterinary Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran 3Department of Microbiology ,Science and Research Branch, Islamic Azad University, Tehran, Iran 4Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Abstract: Alzheimer’s disease (AD) is a neurodegenerative disorder and most common form of dementia that leads to memory impairment. In the present study we have examined the investigating effect of Borago officinale on hipocampla IL-1 Beta protein in the Beta Amyloid peptid induced of inflammation in the rat. Wistar male rats received intrahippocampal (IHP) injection of the Aβ(25–35) and borage extract throughout gestation (100 mg/kg).inflamation was confitmed by pathologist.for mesearing the expression of the IL-1 Beta gene we used RT- PCR method and for extraction of the IL-1 Beta protein we used western bloting method.result showed that using Borago officinale lead to reduce IL-1 Beta gene and protein. This data suggests that borage could improve the reduction of inflammation factors such as IL-1 Beta and borage consumption may lead to an improvement of AD-induced cognitive dysfunction.

Keywords: IL1-Beta; Alzheimer; RT-PCR; Western Blot; Amyloid β-Peptide

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