Acid Phosphatase Level in Selected Tissues of Alloxan Induced Diabetic Rabbits following Administration of Aqueous Extract from Unripe Pulp of Carica Papaya.
I. Oloyede OmotadeDepartment of Biochemistry, University of Ado-Ekiti, Ekiti State (Nigeria).
Abstract: The in vivo effect of oral administration of aqueous extract of unripe pulp Carica papaya on acid phosphatase activity in normal and alloxan induced diabetic rabbits was investigated. Both normal and diabetic rabbits were administered 50mg, 100mg and 200mg per kg body weight of the extract consecutively for two weeks. The effects of the aqueous extract of unripe pulp of Carica papaya in test animals were compared with control group which received distilled water alone. Acid phosphatase is a membrane bound marker enzyme. Kidney and liver levels of Acid phosphatase (ACP) increased in a dose related manner in normal rabbits. This may be due to de novo synthesis of enzyme molecules in these organs. Reduction of Acid phosphatase (ACP) activity in kidney of diabetic rabbits is due to leakage of enzyme through altered lysosomal membrane and the release of its component into extracellular environment. The reduced activity of this membrane bound enzyme suggest altered membrane structure and function. Since there is no corresponding increase in Serum enzyme activities, initiation of disease is not established. This observation confirms the validity of the therapeutic use of the extract in the management of diabetes mellitus.
Keywords: Acid phosphatase Carica papaya; de novo synthesis invivo Back to TOC