Anti-Inflammatory Activity of Raphanus sativus L in Acute and Chronic Experimental Models in Albino Rats
Shobha Kamble1, Md. Zubair Ahmed2, S. Ramabhimaiaha3 and Prabhakar Patil11Department of Pharmacology Al-Ameen Medical College, Bijapur, India. 2Department of Pharmacology Prathima Institute of Medical Science, Karimnagar, India. 3Department of Pharmacology Navodaya Medical College, Raichur, Karnataka, India.
Abstract: Inflammation is a localized reaction that produces redness, warmth, swelling, and pain as a result of infection, irritation, or injury. Edema formation, leukocyte infiltration and Granuloma formation represent such components of inflammation. Raphanus sativus L. ‘Radish’ locally known as Fijl, is a Brassicaceous plant of cabbage family which has been reputed for its beneficial medicinal properties. The present study was undertaken to evaluate the anti-inflammatory effect of freshly squeezed Raphanus Sativus leaf and root juice (FRJ) in acute and chronic experimental models in albino rats. The freshly squeezed Raphanus Sativus leaf and root juice ( FRJ ) was studied in carrageenan induced hind paw edema in albino rats and paw volume was measured plethysmomrtrically at 0 and 3 hr after injection of 1% carageenan in right hind paw and compared with control and standard drug, diclofenac. The drug was also investigated in formalin induced oedema model in rats by injecting 2% formalin in right hind paw. Degree of inflammation was measured plethysmomrtrically on day 1 and day 7 and compared with control and standard drug, diclofenac. All the drugs were administerd orally, control group received 4% gum accasia, standard group received diclofenac 10mg/kg, and test groups received FRJ in doses of 3 ml. Both radish leaf juice and root juice significantly (p<0.01) reduced carageenan and formalin induced paw edema in rats but radish leaf juice compared to root juice produced more significant anti-inflammatory effect in both acute and chronic models of inflammation. However the anti-inflammatory effect of radish leaf juice is less than standard drug diclofenac sodium.
Keywords: Raphanus sativus L; Inflammation; Diclofenac; Carageenan; Formalin Back to TOC