Isolation and Identification of Phenol Degrading Bacteria from Mangrove Sediments in the Persian Gulf (Asaluyeh) and their Growth Kinetics Assay
Farshid Kafilzadeh* and Sedigheh MokhtariDepartment of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
Abstract: Phenol and phenolic compounds are the most common pollutants result of petrochemical industries. Their carcinogenic and toxic effects have been recorded on human being. Since chemical methods of removing these contaminants are expensive, applying microorganisms is suitable substitution to remove these compounds. The purpose of this research is to isolate and identify of phenol degrading bacteria from mangrove sediments in the Persian Gulf (Asaluyeh) and study of their growth kinetics and degradation level. Nine samples of mangrove sediments were collected. Isolation of bacteria was done by culture in a mineral salt-medium containing 0.5 gL-1 phenol. The growth ability of bacteria was evaluated by bacteria culture in 0.3, 0.5, 0.7, 0.9, 1, 2 and 3 gL-1 phenol concentrations. Gibbs indicator was used in order to measure the amount of phenol removal. All of isolated bacteria showed high ability in phenol removal so that Pseudomonas putida and Acinetobacter sp were able to grow in 0.9 to 2 gL-1 phenol concentrations. The findings of this research indicated that mangrove sediments have numerous phenol degrading bacteria such as; Pseudomonas putida, Acinetobacter sp, Bacillus thuringiensis, Brevibacterium iodinum, and Staphylococcus aureus.
Keywords: Phenol; Biodegradation; Pseudomonas putida; Acinetobacter sp; Mangrove sediments Back to TOC