Study of Lipase Production by Acinetobacter sp. YMP
Yasmin C. Attar*, Sayali Randive, Sadiccha Patil and Mrudula M. BendigeriDepartment of Microbiology, Rajaram College, Vidyanagar, Kolhapur - 416 004, India
Abstract: The biosurfactant producing Acinetobacter sp. YMP which showed vegetable oil degradation potential on MSM was studied for lipase production. The production of lipase was confirmed on fat agar & lipase activity was measured by titrimetric method. The effect of environmental factors (agitation, time span , substrate concentration , pH & temperature ) on lipase production was studied by using Acinetobacter sp. YMP. The enzyme was further purified by Ammonium sulphate (60%) precipitation technique. The maximum lipase production by Acinetobacter sp. YMP was found to be within 120 hours at 450 rpm, 3.0 pH , 27° C temperature & at 2% substrate concentration. This species was also able to show upto 8 % oil degradation potential on MSM broth & agar.
Keywords: Biosurfactant; MSM (Mineral salt medium); Lipase Back to TOC