Assessment of Immunocompetence Status of Native Breed of Chickens
R. Saxena1, R. Stephan2, S. K.Mishra4, S. Shukla3, D. P.Saxena1, S. O. Pratap4 and D. P.Singh41Vinayaka Mission University, Salem, T.N., India.
2Govt. Arts College, Department of Botany, Ariyaloor, T.N, India.
3Integral University, Lucknow, U.P., India.
4Central Avian Research Institute, Izatnagar, Bareilly, U.P., India.
Abstract: Immunocompetence is known to be controlled by genetics depending on the B-haplotype Major Histocompatibility complex (MHC), for instance and the selection for production traits. Genetic resistance to disease involves many facets of the body’s defense system and interactions, and it is extremely complex. Production traits had negative correlation to disease resistant traits. The aim of the current study was to estimate the immune responses against SRBC in selected lines, estimating the genetic trend and genetic correlation between male and female immune responses against SRBC. Humoral response was estimated in the form of titer against sheep RBC, each birds was received an intravenous injection of 0.1 ml of 0.5% suspension of packed SRBC in normal saline at 6 week of age Five day later the blood was collected from Jugular vein of each birds in the individual test tube and sera were collected. In the present investigation, chicks belonging to the selected lines of native chickens were used for the study of immunological traits viz., response to sheep RBCs, IgG concentrations. Finally, 1% sheep RBC suspension was prepared by mixing 1 ml of packed sheep RBCs and 99 ml of PBS which was then used for injection in the birds. The Average means for HA was observed (7.74±0.109) and IgG (1.94±0.72) in native breed. Sex wise HA results showed that male have higher mean (7.925 ± 0.1463) than females (7.57± 0.161) for HA while the same was also estimated for IgG by having mean (2.1194±8.9) and female (1.77±8.18). Test of Homogeneity of Variances was recorded as (0.39) for HA which was significant and IgG level (0.937).
Keywords: Immune competence; native breed; HA and HI titer; IgG Back to TOC