Novel Extractive Ultraviolet Spectrophotometric Determination of Serum Simvastatin Concentration
Ndubuisi N. Nwobodo¹*, Paul O. Okonkwo² and Nicholas C. Obitte³¹Department of Pharmacology and Therapeutics, Ebonyi State University (Abakaliki). ²Department of Pharmacology and Therapeutics, University of Nigeria Teaching Hospital, Ituku (Ozalla). ³Department of Pharmaceutical Chemistry, University of Nigeria (Nsukka).
Abstract: This study is aimed at developing and describing a simple, precise, reliable and novel spectrophotometric technique for the determination of serial serum concentration of simvastatin in vivo. Double beam ultraviolet spectrophotometer is employed to determine absorbance readings for corresponding serial concentration of standard stock solution. The absorbance of test sample obtained from serum is then determined and the concentration of test sample deduced from the calibration curve. The calibration curve of simvastatin is found to be linear within Beer-Lambert’s law limits at concentration range of 0.1-20µg/ml. This is represented mathematically in the linear regression equation, y = 0.0174x-0.016. The maximal absorbance wavelength (lmax) is shown to be 280nm. The spectrophotometric technique described in this study has been shown to be quite appropriate, accurate and highly sensitive in detecting very low serum concentrations of simvastatin in vivo; and can be routinely employed clinically for therapeutic drug monitoring.
Keywords: Absorbance; concentration; simvastatin; therapeutic drug monitoring Back to TOC