Antifungal Activity of the Essential Oil of Eugenia Caryophyllata on Candida Albicans, Aspergillus Niger and Aspergillus Flavus
N. Rahimifard1,2, Sh. Shoeibi1,2*, S.R. Pakzad1,2, H. HajimehdipoOr1,2, O. Sabzevari3, S. AjdarY4, F. Bagheri5 and M. Safaee5¹Food and Drug Laboratory Research center (FDLRC), Tehran Iran.
²Food and Drug Control Laboratories (FDCLs), Ministry of Health (MOH), Tehran Iran.
³Pharmacy faculty, Tehran University of Medical Sciences(TUMS), Tehran Iran.
4Immunology Department, Pasteur Institute, Tehran Iran.
5Microbiology Department, Pharmaceutical sciences branch, Islamic Azad University, Tehran Iran.
Abstract: The composition of the essential oil of Eugenia caryophyllata and its antifungal activity on Candida albicans, Aspergillus niger and Aspergillus flavus fungal strains were studied in Iran. Essential oil from the flowers parts of the plant was obtained by hydrodistillation and analysed by GC and GC-MS. The oil showed high contents of Eugenol, B-caryophyllene and Euggenyl acetate. The MIC was used to evaluate the antifungal activity against Candida albicans ATCC 10231, Aspergillus niger ATCC 9642 and Aspergillus flavus ATCC 9643. Antifungal activity was evaluated for the essential oil and simultaneously for Amphotricin B. Results showed that Eugenia caryophyllata essential oil exhibited a significant activity against fungi, and its MIC on Candida albicans, Aspergillus niger and Aspergillus flavus were respectively 0.50, 0.125 and 0.25 µg ml-1 (ppm).The present study indicates that Eugenia caryophyllata essential oil has considerable antifungal activity, deserving further investigation for clinical applications.
Keywords: Eugenia caryophyllata; MIC(minimal inhibitory concentration); Antifungal activity Back to TOC