Evaluation of Antidiabetic Activity of Aqueous Extract of Mangifera Indica Leaves in Alloxan Induced Diabetic Rats
A. Sudha Madhuri and Rajalakshmi Mohanvelu  

Department of Pharmacology, Navodaya Medical College, Raichur Pin Code: 584103  State: Karnataka . Country: India.

Corresponding Author E-mail: allellisudhamadhuri@gmail.com

Abstract: Plants and their bioactive constituents are used for the treatment of diabetes mellitus throughout the world; especially in countries where access to the conventional treatment of diabetes mellitus is inadequate. There have been several reports on the hypoglycemic activity of Mangifera indica(MI). Hence, the present study was undertaken to investigate the anti-diabetic effect of MI leaves extract alone and in combination with  an oral hypoglycemic drug, gliclazide on alloxan induced diabetic rat models. Alloxan monohydrate (125mg/kg, I.P) was used to induce diabetes in albino rats weighing 200- 250 g. The fasted diabetic rats were divided in to 6 groups of 6 animals each. The control group received vehicle (5% gum acacia) only. Group 2 and 3received MI 200mg/kg, 400mg/kg respectively; Group 4 and 5 received Gliclazide 2mg/kg and 4mg/kg respectively and group 6 received MI leaves extract 200mg/kg + Gliclazide 2mg/kg. This study was conducted over a period of 15 days with oral administration of drugs and the plant extract which was started on the 6th day of alloxan treatment. The fasting blood glucose levels will be determined on day 0, 10, and 15th day by using glucometer. Data were statistically analyzed by ANOVA followed by Dunnet’s multiple comparison test. All the test groups showed significant (P < 0.0001) decreased fasting blood glucose levels in alloxan induced diabetic rats treated with the aqueous extract of MI. However, the Combination of MI extract with gliclazide produced a significant (P<0.0001) decrease in blood glucose level which is higher than that produced by gliclazide alone. The decrease in blood glucose levels is probably due to reduction in the intestinal absorption of glucose. MI possesses significant hypoglycemic activity probably due to presence of flavonoids, tannins, steroids and trepenoids and the aqueous extract of leaves contain higher levels of phenol and flavonoids which exhibit greater antioxidant activity by lowering the diabetic complication.

Keywords: Type 2 Diabetes mellitus; albino rats; alloxan monohydrate; Mangifera indica(MI); Gliclazide; Hypoglycemia

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