Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and P53, in T47D Breast Cancer Cells
Muhartono1, Asep Sukohar1, Sutyarso2 and Mohammad Kanedi21Department of Anatomical Pathology, Faculty of Medicine, University of Lampung, Indonesia.
2Department of Biology, Faculty of Mathematics and Sciences, University of Lampung, Indonesia.
Corresponding Author E-mail: wegayendi@yahoo.com
Abstract: Though mucoxin has manufactured and widely marketed online, but indepth study of the role of these annonaceous substances on cellular level is still limited.This study aims to find out more about mucoxin anti-cancer effects, especially those associated with the expression of pro-apoptotic proteins, Bax and p53 in T47D breast cancer cell line.The T47D cell lines was divided into four groupsbased on the hour of mucoxin exposure until assaysdone, namely 0, 24, 48, and 72 hours.Concentration levels of mucoxin applied in theexperiment were 0ng/ml, 0.1ng/ml, 0.5ng/ml, 1ng/ml, 5ng/ml and 10ng/ml. The bax gene expression assayed using quantitative PCR (qPCR) methods, whereas protein expression of bax and p53 determined by immunocytochemistry techniques. The results showed that in comparison with the control, both bax and p53 proteins increase by mucoxin treatment.It is suggested that mucoxin worthy classified as a promising anti-cancer drug.
Keywords: Mucoxin; acetogenin; p53; bax; pro-apoptosis protein; T47D cells; protein expression Back to TOC